Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
BBa_J61101BBa_J61101 Version 1 (Component)Ribosome Binding Site Family Member
EcoRIEcoRI Version 1 (Component) NheINheI Version 1 (Component) Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
PacIPacI Version 1 (Component) SpeISpeI Version 1 (Component) BBa_J61120BBa_J61120 Version 1 (Component)Ribosome Binding Site Family Member
BBa_K2066504BBa_K2066504 Version 1 (Component)ICA:BsmBI Cut Site + G + Sticky End 2
BBa_K2066501BBa_K2066501 Version 1 (Component)ICA:BsmBI Cut Site + G + Sticky End 1
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
BBa_K2066018BBa_K2066018 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
BBa_J15001BBa_J15001 Version 1 (Component)strong synthetic E. coli ribosome binding site with SacI site.
hixCBBa_J44000 Version 1 (Component)hixC binding site for Salmonella typhimurium Hin recombinase
BBa_K2066505BBa_K2066505 Version 1 (Component)BsmBI Cut Site + G + Sticky End 3
BBa_J61100BBa_J61100 Version 1 (Component)Ribosome Binding Site Family Member
BBa_J85600BBa_J85600 Version 1 (Component)attB1 recombination site
CatBBa_I14033 Version 1 (Component)P(Cat)
BBa_I13457BBa_I13457 Version 1 (Component)Improved insertion site with two RNAse E cut sites
pT7BBa_K525998 Version 1 (Component)Promoter T7 and RBS
cI+luxRBBa_R0065 Version 1 (Component)Promoter (lambda cI and luxR regulated -- hybrid)
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_J52008BBa_J52008 Version 1 (Component)luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
BBa_K299107BBa_K299107 Version 1 (Component)T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)
BBa_K1763004BBa_K1763004 Version 1 (Component)Major Spidroin Protein 2 (MaSp2) with sticky ends CA
BBa_K1114107BBa_K1114107 Version 1 (Component)Bicistronic Design RBS 2, MoClo Format with BC fusion sites
RBS_cassetteRBS_cassette Version 1 (Component)AvrII restriction site (cctagg), overlapped with RBS (aggagga)
"G"BBa_K1080009 Version 1 (Component)ChlG
BBa_K1763009BBa_K1763009 Version 1 (Component)Major Spidroin Protein 2 (MaSp2) Sequencing Core with AB Sticky Ends
loxBBa_J61046 Version 1 (Component)[Lox] site for recombination
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
RBS-spoVGBBa_K143021 Version 1 (Component)SpoVG ribosome binding site (RBS) for B. subtilis
BBa_I13452BBa_I13452 Version 1 (Component)Adds another BioBrick site
BBa_I13455BBa_I13455 Version 1 (Component)Insertion site plus RNAse E cut site
BBa_K165002BBa_K165002 Version 1 (Component)Kozak sequence (yeast RBS)
PBS_F24PBS_F24 Version 1 (Component) BBa_K1763010BBa_K1763010 Version 1 (Component)Major Spidroin Protein 1 (MaSp1) with Sticky Ends AB
BBa_K1763012BBa_K1763012 Version 1 (Component)Major Spidroin Protein 1 (MaSp1) with Sticky Ends CA
BBa_K1763011BBa_K1763011 Version 1 (Component)Major Spidroin Protein 1 (MaSp1) with Sticky Ends BC
T7 RBSBBa_K1362090 Version 1 (Component)strong T7 RBS