BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1831000
BBa_K1831000 Version 1 (Component)
NpuDnaE intein for circularization of Type lll AFP+ inker

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

BBa_K300084
BBa_K300084 Version 1 (Component)
Phasins with intein downstream (separated by a flexible protein domain linker)

BBa_K323105
BBa_K323105 Version 1 (Component)
Zif268 zinc finger + linker

BBa_K249008
BBa_K249008 Version 1 (Component)
YFP with N-terminal Arganine Tag

BBa_I732017
BBa_I732017 Version 1 (Component)
lacZ full-length (RBS+ TERM-)

BBa_K081009
BBa_K081009 Version 1 (Component)
lasI protein generator (TERM-)

BBa_K300095
BBa_K300095 Version 1 (Component)
Phasin and intein separated by a flexible protein domain linker

BBa_K1051704
BBa_K1051704 Version 1 (Component)
Mer2 Intron

LacI
BBa_K143033 Version 1 (Component)
LacI (Lva^-, N-terminal deletion) regulatory protein

BBa_K516030
BBa_K516030 Version 1 (Component)
mRFP protein generator TERM+ RBS+(B0030)

BBa_K081008
BBa_K081008 Version 1 (Component)
luxI protein generator (TERM-)

SspDnaE(N)
BBa_K1362150 Version 1 (Component)
RBS + SspDnaE N-intein RFC[105] assembly construct (with His6)

Stop
BBa_K1362473 Version 1 (Component)
C terminal Stop insert for RFC[???] N intein constructs with two insertion sites

BBa_K1362474
BBa_K1362474 Version 1 (Component)
N terminal Gly insert for RFC[???] C intein constructs with two insertion sites

BBa_K2017000
BBa_K2017000 Version 1 (Component)
C-split Cas9 + DnaE C-intein

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

5xGS
BBa_J18922 Version 1 (Component)
10aa [GS]x linker

BBa_K1486004
BBa_K1486004 Version 1 (Component)
flexible linker 2x (GGGGS)

BBa_K1859003
BBa_K1859003 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +HHHHHH linker

BBa_K1859001
BBa_K1859001 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GGSGGS linker

BBa_K1859004
BBa_K1859004 Version 1 (Component)
GGSGGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1859006
BBa_K1859006 Version 1 (Component)
HHHHHH linker+ The 5??side of the intron [BBa_K1332003]

BBa_K1362460
BBa_K1362460 Version 1 (Component)
RGK (extein)

BBa_K1362051
BBa_K1362051 Version 1 (Component)
Intein protease with arabinose inducible regulatory promoter/ repressor araC-Pbad-T7RBS-His-SspDnaBC

BBa_K1638036
BBa_K1638036 Version 1 (Component)
Sce VMA Intein with promoter

BBa_K300083
BBa_K300083 Version 1 (Component)
Phasins (separated by a flexible protein domain linker) with intein downstream

BBa_K1362108
BBa_K1362108 Version 1 (Component)
RBS + NpuDnaE N&C-intein coexpression RFC[105] assembly construct (with His6)

linker
BBa_K365005 Version 1 (Component)
20 aa linker RFC-25 (fusion standard Freiburg)

BBa_K1459011
BBa_K1459011 Version 1 (Component)
PmrB(N-term)

BBa_K1362109
BBa_K1362109 Version 1 (Component)
RBS + NpuDnaE N&C-Intein coexpression RFC[105] nonsplicing assembly construct (with His6)

BBa_K1638035
BBa_K1638035 Version 1 (Component)
hTrx-based scaffold fused to T18 through intein and a flexible linker

BBa_J52026
BBa_J52026 Version 1 (Component)
dnMyD88-linker-GFP

BBa_K876060
BBa_K876060 Version 1 (Component)
Term-Term-plasR-RBS-CFP-Term-Term