BBa_K233306
BBa_K233306 Version 1 (Component)
YcdB - This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT)

BBa_J58004
BBa_J58004 Version 1 (Component)
Robins temporary part for test purposes

BBa_K200006
BBa_K200006 Version 1 (Component)
OtsB: Part 2 of 2 coding for trehalose producing enzymes

BBa_J70435
BBa_J70435 Version 1 (Component)
Forward oligo part for testing J70428, J70429, J70430

Paro
BBa_K1884000 Version 1 (Component)
Paromomycin

LSSL
BBa_K2026000 Version 1 (Component)
LSSL, a composite part acts as genetic switch

BBa_K182003
BBa_K182003 Version 1 (Component)
Intermediate part consisting of CI promoter followed by tetR, LacI and Tet Operator

BBa_K228003
BBa_K228003 Version 1 (Component)
Bistable part

BBa_M1659
BBa_M1659 Version 1 (Component)
Composite part for lung cancer biomarker

BBa_J70394
BBa_J70394 Version 1 (Component)
J04450 {0,5:5,0} part

BBa_J34710
BBa_J34710 Version 1 (Component)
XOR Part1

BBa_J34711
BBa_J34711 Version 1 (Component)
XOR Part2

BBa_J34712
BBa_J34712 Version 1 (Component)
XOR Part3

BBa_K576003
BBa_K576003 Version 1 (Component)
Left Part of Self-Excising Ribozyme

BBa_K576004
BBa_K576004 Version 1 (Component)
Right Part of Self-Excising Ribozyme

BBa_K145016
BBa_K145016 Version 1 (Component)
Intermediate TetR-generator

BBa_K1937002
BBa_K1937002 Version 1 (Component)
OriKan: a composite part to turn any pSB1C3 into a <i>Bacillus</i> plasmid

BBa_J06499
BBa_J06499 Version 1 (Component)
Construction Intermediate IC

BBa_K546546
BBa_K546546 Version 1 (Component)
The Synchroscillator: A Tunable Synchronized Oscillatory System

BBa_K1713000
BBa_K1713000 Version 1 (Component)
Plac/ara-1, a hybrid promoter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K539641
BBa_K539641 Version 1 (Component)
a RBS (B0032)+ilvC with a RBS (B0032)+ilvD

EYFP
BBa_E0036 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ECFP
BBa_E0026 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_I12008
BBa_I12008 Version 1 (Component)
Barkai-Leibler design experiment part A (p22cII)

BBa_K1513000
BBa_K1513000 Version 1 (Component)
A quorum sensing system which can produce auto-inducer (AHL) itself.

BBa_K209408
BBa_K209408 Version 1 (Component)
AarI C-D part, Intersectin (ITSN)

BBa_K209411
BBa_K209411 Version 1 (Component)
AarI C-D part, Lamellipodin (LPD) (775-1250)

BBa_K539621
BBa_K539621 Version 1 (Component)
a RBS (B0030)+ilvC

BBa_K1945002
BBa_K1945002 Version 1 (Component)
Homologous Recombination Intermediate for Counter-selection in Synechocystis sp. PCC 6803

BBa_J329006
BBa_J329006 Version 1 (Component)
Simple QS Circuit with GFP Controlled by LuxR Promoter

BBa_K173007
BBa_K173007 Version 1 (Component)
aTc inducible system (aTc->PoPS) with J23100 promoter

BBa_K209015
BBa_K209015 Version 1 (Component)
Aar1 C-D part, Inp54p

BBa_K2055016
BBa_K2055016 Version 1 (Component)
Part designed for verification and characterization of the FUR/LacI inverter

FUS1 pr
BBa_K1154001 Version 1 (Component)
Saccharomyces cerevisiae FUS1 promoter (induced by mating pheromone)

BBa_K323005
BBa_K323005 Version 1 (Component)
CMV ATG PBSII link nYFP stop - part of mammalian split/FRET system

BBa_K323021
BBa_K323021 Version 1 (Component)
CMV ATG Gli1 link nCFP stop - part of mammalian split/FRET system

BBa_K209409
BBa_K209409 Version 1 (Component)
AarI C-D part, Tuba

BBa_K174001
BBa_K174001 Version 1 (Component)
Arabinose inducible system

BBa_K987000
BBa_K987000 Version 1 (Component)
This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p

BBa_J70437
BBa_J70437 Version 1 (Component)
Reverse oligo part for testing J70428, J70429, J70430

BBa_J70436
BBa_J70436 Version 1 (Component)
Forward oligo part for testing J70428, J70429, J70430

BBa_K748000
BBa_K748000 Version 1 (Component)
AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.

ChiPm-lacZ
BBa_K564013 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_J95032
BBa_J95032 Version 1 (Component)
Terminator measurement part 1 for R. sphaeroides

BBa_J95033
BBa_J95033 Version 1 (Component)
Terminator measurement part 2 for R. sphaeroides

"Part X"
BBa_K1001752 Version 1 (Component)
RFP and RBS behind Lamda Prm Promoter

DesR-A
BBa_I732002 Version 1 (Component)
DesR-A (LVA-)

BBa_K209487
BBa_K209487 Version 1 (Component)
AarI B-D part, DOR-EzrinABDT