BBa_K1113411BBa_K1113411 Version 1 (Component)Targeting sequence for the delivery of the LacZ gene to the Carboxysome
BBa_K1109007BBa_K1109007 Version 1 (Component)FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence transc
BBa_K1778005BBa_K1778005 Version 1 (Component)eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene
CP1LacPI2BBa_K418002 Version 1 (Component)IPTG inducible Lac promoter cassette
BBa_I12026BBa_I12026 Version 1 (Component)Test of BBa_R0011 (LacI regulated) using YFP
BBa_K1461323BBa_K1461323 Version 1 (Component)pEpCAM(mutant)-RBS-GFP-2 miR 142 3p non-bindgin sites-2 miR 142 5p non-binding site-d.term
BBa_K188122BBa_K188122 Version 1 (Component)RBS+lacI repressor from E. coli (+LVA)
BBa_K1321362BBa_K1321362 Version 1 (Component)sfGFP fused to CBDcex driven by LacI
BBa_K737000BBa_K737000 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K1472613BBa_K1472613 Version 1 (Component)∆9, ∆12, ∆15 desaturases with LacI promoter
BBa_M45102BBa_M45102 Version 1 (Component)Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese
BBa_K737001BBa_K737001 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_I715071BBa_I715071 Version 1 (Component)Lac promoter with Ribosomal Binding Site
BBa_K1676120BBa_K1676120 Version 1 (Component)Mutant 16 of Lactate Dehydrogenase
BBa_K1676114BBa_K1676114 Version 1 (Component)Mutant 10 of Lactate Dehydrogenase
BBa_K639004BBa_K639004 Version 1 (Component)rrnB P1-LacI-pLac-mCherry plausible stress sensor
BBa_K1615108BBa_K1615108 Version 1 (Component)mRFP fused to CBDclos driven by LacI promoter
BBa_K549004BBa_K549004 Version 1 (Component)LacI promotor fused with the iron dependent regulator fur
BBa_K329042BBa_K329042 Version 1 (Component)Right arm Tn916 - Wild Type + Mutant Lambda
IHGBBa_K1995024 Version 1 (Component)IHG (INP-N-GFP-HG)
BBa_K1452000BBa_K1452000 Version 1 (Component)Pos/neg selection (Bacillus)
BBa_J47053BBa_J47053 Version 1 (Component)Constitutive device (medium transcription) for lacI repressor, strong RBS
BBa_K132016BBa_K132016 Version 1 (Component)luxI+KanR-LVA+LacI+PL+KanR-LVA+aiiA+terminator
BBa_K418001BBa_K418001 Version 1 (Component)From partsregistry.org IPTG inducible Lac promoter cassette
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
FBS-AceB+LBBa_K1163999 Version 1 (Component)Inverter composed of Fur Binding site from AceB promoter + LacI-LVA
BBa_K584011BBa_K584011 Version 1 (Component)Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP
BBa_J40000BBa_J40000 Version 1 (Component)Quorum sensing promoter with lac I and CFP
BBa_K1222004BBa_K1222004 Version 1 (Component)pCam(T7 promoter+lac operator+CamR antisense 2+T7 terminator)
BBa_K1195012BBa_K1195012 Version 1 (Component)T7 Bacteriophage Minor Capsid Protein (Large Mutant 2)
BBa_K1676088BBa_K1676088 Version 1 (Component)Mutant 12 of LDH and wildtype LDP
BBa_K1676091BBa_K1676091 Version 1 (Component)Mutant 15 of LDH and wildtype LDP
BBa_K1676086BBa_K1676086 Version 1 (Component)Mutant 10 of LDH and wildtype LDP
BBa_K1676080BBa_K1676080 Version 1 (Component)Mutant 4 of LDH and wildtype LDP
BBa_K1362300BBa_K1362300 Version 1 (Component)NpuDnaE N-Intein RFC[105] assembly construct (with His6)
BBa_K726009BBa_K726009 Version 1 (Component)T7 driven lac operated inducer for the rhl quorum-sensing system
BBa_M50056BBa_M50056 Version 1 (Component)PETase double mutant I208V and R90A full plasmid information
BBa_K079021BBa_K079021 Version 1 (Component)LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La
BBa_J22121BBa_J22121 Version 1 (Component)Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3
BBa_I758600BBa_I758600 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_K1154006BBa_K1154006 Version 1 (Component)Mating pheromone-induced IGPD and constitutive LDH expression in yeast
BBa_K1520509BBa_K1520509 Version 1 (Component)PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
BBa_K346025BBa_K346025 Version 1 (Component)PmerT promoter mutant 88+RBS(B0030)+GFP(E0040)Terminator(B0010)+Terminator(B0012)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.