BBa_K080004
BBa_K080004 Version 1 (Component)
Alpha Cardiac Myosin Heavy Chain minPro ??? Neomycin Resistance ??? fmdv2a-GFP

BBa_K891008
BBa_K891008 Version 1 (Component)
Probe coding for alpha fragment of beta gal 2

BBa_K195203
BBa_K195203 Version 1 (Component)
RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator

BBa_K1321300
BBa_K1321300 Version 1 (Component)
pSEVA331-Bb - pSEVA331 with biobrick prefix and suffix

BBa_K1969006
BBa_K1969006 Version 1 (Component)
S. cerevisiae GPA2 with 5 amino acids from human G(s) alpha-subunit

BBa_K539733
BBa_K539733 Version 1 (Component)
kivd (alpha-ketoisovalerate decarboxylase) with native rbs(BBa_B0034) and terminator

BBa_K539732
BBa_K539732 Version 1 (Component)
kivd (alpha-ketoisovalerate decarboxylase) with native rbs(BBa_B0030) and terminator

BBa_K875007
BBa_K875007 Version 1 (Component)
T5 LacO-PelB-SIP (scFv 54.6 + alpha isotype CH3 antinorovirus)-6HIS-TT

BBa_K875005
BBa_K875005 Version 1 (Component)
T5LacO-LPP-OmpA-SIP (scFv 54.6 + alpha isotype CH3 antinorovirus)-6HIS-TT

BBa_I716069
BBa_I716069 Version 1 (Component)
2X mutant -pTRC-rbs-beta-rbs-alpha L29F/V96W and Asn108Lys/Lys82Asp

BBa_K1906004
BBa_K1906004 Version 1 (Component)
Elongation Factor Tu

BBa_K112214
BBa_K112214 Version 1 (Component)
{< ihfA!} The ihf alpha gene without a start codon, BBb format

BBa_K112215
BBa_K112215 Version 1 (Component)
{ihfA>} The ihf alpha gene without a stop codon, BBb format

BBa_K539741
BBa_K539741 Version 1 (Component)
TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_J61048)

BBa_K1906000
BBa_K1906000 Version 1 (Component)
Elongation Factor Ts

BBa_K891002
BBa_K891002 Version 1 (Component)
Probe coding for alpha fragment of beta gal (long linker)

BBa_K891006
BBa_K891006 Version 1 (Component)
Probe coding for alpha fragment of beta gal (long linker)

BBa_K195204
BBa_K195204 Version 1 (Component)
pTet+RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator

BBa_J64100
BBa_J64100 Version 1 (Component)
Medium copy ColE1 BioBrick vector

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Spo0A
BBa_J01004 Version 1 (Component)
Spo0A Transcription Factor

BBa_K2143001
BBa_K2143001 Version 1 (Component)
NahR_L154F Transcription Factor

BBa_K362004
BBa_K362004 Version 1 (Component)
sodA promoter is controlled by some transcription factors including SoxS.

BBa_K1714005
BBa_K1714005 Version 1 (Component)
pBad/araC-RBS B0034-Ag43 thanatin 2mer as alpha domain-double terminator

BBa_K1714007
BBa_K1714007 Version 1 (Component)
pBad/araC-RBS B0034-Ag43 thanatin 4mer as alpha domain-double terminator

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K1714006
BBa_K1714006 Version 1 (Component)
pBad/araC-RBS B0034-Ag43 thanatin 3mer as alpha domain-double terminator

BBa_J329002
BBa_J329002 Version 1 (Component)
Intermediate part for making a QS system

BBa_K1054003
BBa_K1054003 Version 1 (Component)
lrgB antiholin factor

BBa_M36151
BBa_M36151 Version 1 (Component)
nifA transcription factor

BBa_M36251
BBa_M36251 Version 1 (Component)
AlkS Transcription Factor

BBa_K1039013
BBa_K1039013 Version 1 (Component)
PhiC31 Recombination Directionality Factor

BBa_M36020
BBa_M36020 Version 1 (Component)
Transcription Factor lldR

victor
BBa_I714005 Version 1 (Component)
abc

BBa_K353001
BBa_K353001 Version 1 (Component)
AfsR Transcription Factor

BBa_K2110017
BBa_K2110017 Version 1 (Component)
&#945;-factor signal peptide

pSB1AK3
pSB1AK3 Version 1 (Component)
High copy BioBrick assembly plasmid

BBa_K541515
BBa_K541515 Version 1 (Component)
Limulus anti-LPS factor (LALF) for Bacillus subtilis

BBa_K258010
BBa_K258010 Version 1 (Component)
Human Epidermal growth factor (hEGF)

BBa_K353007
BBa_K353007 Version 1 (Component)
Regulatory transcription factor

comP
BBa_K233304 Version 1 (Component)
Competence factor P

BBa_K1054024
BBa_K1054024 Version 1 (Component)
lrgA antiholin factor

BBa_M36798
BBa_M36798 Version 1 (Component)
Stress-regulated transcription factor

BBa_K142040
BBa_K142040 Version 1 (Component)
ribosome modulation factor (RMF)

com C
BBa_K233302 Version 1 (Component)
competence factor C , Acinetobacter BD413

BBa_K896902
BBa_K896902 Version 1 (Component)
Against aging!!

BBa_J23006
BBa_J23006 Version 1 (Component)
Part-making plasmid with Ptet-XbaI-[biobrick]-SpeI-PstI

SinR
BBa_K1122000 Version 1 (Component)
SinR transcription factor

BBa_K1039006
BBa_K1039006 Version 1 (Component)
Bxb1 Recombination Directionality Factor

BBa_K209473
BBa_K209473 Version 1 (Component)
pcDNA3.1(+) with AarI A-D sites in MCS