Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K199114
BBa_K199114 Version 1 (Component)
TT-pLux-RBS

BBa_K199103
BBa_K199103 Version 1 (Component)
pLac/pL-RBS-LuxR-TT

BBa_K1529291
BBa_K1529291 Version 1 (Component)
Plux/lac_aiiA

BBa_K199111
BBa_K199111 Version 1 (Component)
pLux-RBS

BBa_K1529290
BBa_K1529290 Version 1 (Component)
Plux/lac_luxI

BBa_K189018
BBa_K189018 Version 1 (Component)
L of lambda genome

BBa_K189019
BBa_K189019 Version 1 (Component)
M of lambda genome

BBa_K189013
BBa_K189013 Version 1 (Component)
G of lambda genome

BBa_K1323999
BBa_K1323999 Version 1 (Component)
RBS of a part

BBa_K189010
BBa_K189010 Version 1 (Component)
Fi of lambda genome

pMam
BBa_K678023 Version 1 (Component)
Plasmid-Mam for Plug

BBa_K1725215
BBa_K1725215 Version 1 (Component)
LuxC + LuxD with BBa_K1725080 lac regulated promoter

CreA
BBa_I715000 Version 1 (Component)
Amino Half of Cre Recombinase (aka CreA)

CreB
BBa_I715001 Version 1 (Component)
Carboxl Half of Cre Recombinase (aka CreB)

BBa_K1585321
BBa_K1585321 Version 1 (Component)
glgCAB for polycistronic expression of glycogen synthesis genes in E.coli

BBa_K1947022
BBa_K1947022 Version 1 (Component)
Improve the protein purification.

BBa_K327016
BBa_K327016 Version 1 (Component)
Lux activated, C1lam repressed switch

BBa_K1351001
BBa_K1351001 Version 1 (Component)
Contactin-4-derived peptide binding to NADH Oxidase of S. pneumoniae

BBa_K078005
BBa_K078005 Version 1 (Component)
2，2???，3-Trihydroxybiphenyl 1,2-dioxygenase. The second step enzyme in dixon degradati

BBa_I751501
BBa_I751501 Version 1 (Component)
plux-cI hybrid promoter

BBa_K1114100
BBa_K1114100 Version 1 (Component)
MoClo format of a modified version of BBa_B0030 with BC fusion sites.

BBa_K1114102
BBa_K1114102 Version 1 (Component)
MoClo format of a modified version of BBa_B0032 with BC fusion sites

BBa_K1114101
BBa_K1114101 Version 1 (Component)
MoClo format of a modified version of BBa_B0031 with BC fusion sites.

BBa_K1351018
BBa_K1351018 Version 1 (Component)
Promoter for the sdpIR operon

BBa_K1431101
BBa_K1431101 Version 1 (Component)
TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA

BBa_K1913005
BBa_K1913005 Version 1 (Component)
lux quorum sensing system + GFP reporter

BBa_J69801
BBa_J69801 Version 1 (Component)
Lux Receiver with inducible aiiA Device

BBa_K584013
BBa_K584013 Version 1 (Component)
Lac-Lux hybrid promotor + Ribolock-CeaB

BBa_K199116
BBa_K199116 Version 1 (Component)
RBS-RFP-TT-pLac-RBS-LuxR-TT

BBa_K1926012
BBa_K1926012 Version 1 (Component)
The VIKA UNIT: recombinase VIKA flanked by Vox

BBa_K1631006
BBa_K1631006 Version 1 (Component)
rbs - Immunity protein of Colicin-E3 - d.term

BBa_K1631004
BBa_K1631004 Version 1 (Component)
Tlanslational unit of Immunity protein for Colicin-E3

BBa_K415011
BBa_K415011 Version 1 (Component)
PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI

BBa_K077019
BBa_K077019 Version 1 (Component)
pluxR with cI (lambda) behind a normal RBS

BBa_J100098
BBa_J100098 Version 1 (Component)
Promoter for the argF gene

BBa_K313010
BBa_K313010 Version 1 (Component)
loading sequence of RNA phage MS2

BBa_K1333002
BBa_K1333002 Version 1 (Component)
M13 bacteriophage g3p with start codon of "ATG" and a FLAG tag on C-terminal

BBa_I735010
BBa_I735010 Version 1 (Component)
This promoter encoding for a thiolase involved in beta-oxidation of fatty acids.

BBa_K1555000
BBa_K1555000 Version 1 (Component)
The copa promoter wth a gfp reporter

BBa_K200015
BBa_K200015 Version 1 (Component)
Coding region for the DpnII restriction enzyme.

BBa_K415069
BBa_K415069 Version 1 (Component)
pLux-Lac:GFP:Term:Term:PluxR/cI-OR :mCherry:Term:PtetR:LuxR:Term:Fixed-pLux/cI:LuxI

BBa_J22121
BBa_J22121 Version 1 (Component)
Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3

pir
BBa_I716102 Version 1 (Component)
pir (Induces the R6K Origin)

BBa_K153008
BBa_K153008 Version 1 (Component)
CqsA - the CAI-1 producer

BBa_K182102
BBa_K182102 Version 1 (Component)
pLux-Lac with RBS attached

BBa_K896906
BBa_K896906 Version 1 (Component)
this is a hormone produced mainly in guts used for controlling the appetite

BBa_J100101
BBa_J100101 Version 1 (Component)
High production of eCDM8

BBa_K611093
BBa_K611093 Version 1 (Component)
A Truncation of BBa_C0051