BBa_K737040
BBa_K737040 Version 1 (Component)
galK::GFP generator ligated to TetR generator and constitutive sRNA device

BBa_K1956013
BBa_K1956013 Version 1 (Component)
gRNA for Kanamycin resistance gene under lambda pL hybrid promoter with terminator

BBa_K382069
BBa_K382069 Version 1 (Component)
intron hairpin RNAi knockdown of Bet v 1 allergen in Kan plant vector

BBa_J64852
BBa_J64852 Version 1 (Component)
DNA stretch between OmpR sigma 70 promoter -10 site and start of transcription

BBa_K382068
BBa_K382068 Version 1 (Component)
intron hairpin RNAi knockdown of Bet v 1 allergen in Basta plant vector

BBa_K1124104
BBa_K1124104 Version 1 (Component)
clock device (plux/tet) (-luxI, -tetR, -aiiA, --sRNA, +amilGFP)(AHL sensor)

BBa_K2050422
BBa_K2050422 Version 1 (Component)
Mouse H1 promoter (RNA Polymerase III promoter)

BBa_K1363604
BBa_K1363604 Version 1 (Component)
key of no of RNA logic gates

BBa_K737050
BBa_K737050 Version 1 (Component)
The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42

BBa_K1124102
BBa_K1124102 Version 1 (Component)
clock device (plux/tet) (+luxI, -tetR, -aiiA, --sRNA, +amilGFP)(positive feedback only)

BBa_K1178000
BBa_K1178000 Version 1 (Component)
tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon

BBa_K1124103
BBa_K1124103 Version 1 (Component)
clock device (plux/tet) (-luxI, +tetR, +aiiA, ++sRNA, +amilGFP)(negative feedback only)

BBa_K1179017
BBa_K1179017 Version 1 (Component)
A mutant DNA binding Cas9 with an N-terminal linker and BsaI site for ligation

BBa_K1179008
BBa_K1179008 Version 1 (Component)
A mutant DNA binding Cas9 with a C-terminal linker and BsaI site for ligation

BBa_K896599
BBa_K896599 Version 1 (Component)
Strophurus rankini voucher AMS R140490 NADH dehydrogenase subunit 4 (ND4) gene, partial cds; tRNA-Hi

iGEM 2019 Plates
iGEM_2019_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2019

iGEM 2018 Plates
iGEM_2018_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2018

BBa_K1124100
BBa_K1124100 Version 1 (Component)
clock device (plux/tet) (+luxI, +tetR, +aiiA, ++sRNA, +amilGFP, +UV reset device(E234K, -LVA)

BBa_K880001
BBa_K880001 Version 1 (Component)
Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K

BBa_K323151
BBa_K323151 Version 1 (Component)
DNA program 123456

BBa_K323152
BBa_K323152 Version 1 (Component)
DNA Program 12346

BBa_K801032
BBa_K801032 Version 1 (Component)
GAL4 DNA binding domain

BBa_K105101
BBa_K105101 Version 1 (Component)
Gal4 - DNA binding domain

BBa_K105100
BBa_K105100 Version 1 (Component)
LacI - DNA binding domain

BBa_K887011
BBa_K887011 Version 1 (Component)
DNA program : specific DNA sequence which can be recognized by zinc fingers

BBa_K199091
BBa_K199091 Version 1 (Component)
pLac-CUACC Suppressor tRNA

BBa_K779120
BBa_K779120 Version 1 (Component)
RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488) MammoBlock

BBa_J119088
BBa_J119088 Version 1 (Component)
pT7-RBS-GFP-tRNA (K191)

BBa_J119087
BBa_J119087 Version 1 (Component)
pT7-RBS-GFP-tRNA (K191)

CmsRNA
BBa_K1960102 Version 1 (Component)
sRNA, which can silence chloramphenicol resistance

BBa_K199170
BBa_K199170 Version 1 (Component)
T7 Promoter RBS RFP pLac CCACC (tRNA)

BBa_K199169
BBa_K199169 Version 1 (Component)
T7 Promoter RBS RFP pLac CUAGC (tRNA)

BBa_K187254
BBa_K187254 Version 1 (Component)
folD, ORF, forward primer

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K809710
BBa_K809710 Version 1 (Component)
GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator

BBa_K779122
BBa_K779122 Version 1 (Component)
Short DNA reporter bottom strand (with ROX fluorophore) MammoBlock

BBa_K779121
BBa_K779121 Version 1 (Component)
Short DNA reporter top strand (with RQ quencher) MammoBlock

PchiP-lacZ
BBa_K564002 Version 1 (Component)
Chitoporin fused with lacZ - target for sRNA based regulation

BBa_K199070
BBa_K199070 Version 1 (Component)
I13453:K199016 Pbad promotor with the suppressor tRNA of CUAGC

BBa_K1942000
BBa_K1942000 Version 1 (Component)
A shRNA corresponding DNA sequence for KRAS which could silence the gene

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K199071
BBa_K199071 Version 1 (Component)
I13453:K199014: Pbad promomtor with the suppressor tRNA of the codon AGGAC

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.