BBa_K1696020
BBa_K1696020 Version 1 (Component)
tag6 for mf-lon to recognize

BBa_K1696018
BBa_K1696018 Version 1 (Component)
tag4 for mf-lon to recognize

BBa_K176248
BBa_K176248 Version 1 (Component)
Low input score automatic selector (with reporter): PoPS->mRFP->CI Inverter (cI-LVA+pCI)->K176229

BBa_K1768006
BBa_K1768006 Version 1 (Component)
Lox recombinase terminator excision switch

BBa_J119408
BBa_J119408 Version 1 (Component)
Pupp promoter mutant - Substitution of C and G to A at 28 and 30

BBa_K202004
BBa_K202004 Version 1 (Component)
Hybrid promoter having multiple operator sites. Promoter has tetO2 with mutation at position 3

BBa_K315030
BBa_K315030 Version 1 (Component)
pTet + loxP forward + RBS + RFP + lox 5171 forward

BBa_K1088059
BBa_K1088059 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1088052
BBa_K1088052 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1178000
BBa_K1178000 Version 1 (Component)
tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon

BBa_K833014
BBa_K833014 Version 1 (Component)
constitutive promoter followed by a lox site with a bidirectional terminator and then a lox site.

BBa_I715002
BBa_I715002 Version 1 (Component)
pLac-lox-RBS-Tet (in pSB3K3)

IodoY RS
BBa_K1416001 Version 1 (Component)
The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon

BBa_K987001
BBa_K987001 Version 1 (Component)
This is a composite part which has the function to invert the temperature activation by the part: BB

BBa_K315012
BBa_K315012 Version 1 (Component)
LoxBri (F) Variant forward lox site with 3 base changes in the spacer region

BBa_S03729
BBa_S03729 Version 1 (Component)
RFP(reverse)-TT-lox-RBS-Tet

BBa_K1075018
BBa_K1075018 Version 1 (Component)
J23105-RBS32-SspB[Core]-LOV-ipaA-TT

BBa_K176249
BBa_K176249 Version 1 (Component)
Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240

BBa_K212006
BBa_K212006 Version 1 (Component)
mRBP (modified ribose binding protein - now binds histamine)

BBa_K576012
BBa_K576012 Version 1 (Component)
J23101-RBS-GFP1-Lox-GFP2-Transcription Terminator

BBa_K1087016
BBa_K1087016 Version 1 (Component)
Ptet- reverse lox- reverse PO promoter- mRFP1

BBa_K1075019
BBa_K1075019 Version 1 (Component)
AraC-pBAD-RBS32-SspB[Core]-LOV-ipaA-TT

BBa_K785004
BBa_K785004 Version 1 (Component)
Lov-HTH (light sensor with Ptet repression) light->PtetO repressed

BBa_I711026
BBa_I711026 Version 1 (Component)
Amy is learning how to build a biobrick. Hor.

BBa_K576011
BBa_K576011 Version 1 (Component)
J23101-RBS-GFP1-IN1-Lox-IN2-GFP2-TT

BBa_K1395000
BBa_K1395000 Version 1 (Component)
nrfA gene (Nitrite reductase enzyme) under constitutive promoter

BBa_K258012
BBa_K258012 Version 1 (Component)
AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism

BBa_K1157007
BBa_K1157007 Version 1 (Component)
Quorum sensing receptor pqsR generator

BBa_K1991007
BBa_K1991007 Version 1 (Component)
Pcons-RBS-LO-BamHI

BBa_K315002
BBa_K315002 Version 1 (Component)
variant reverse lox site with 2 base changes in the spacer region (lox5171)

BBa_K1991010
BBa_K1991010 Version 1 (Component)
Pcons-RBS-LO-GFP-His

BBa_K1991005
BBa_K1991005 Version 1 (Component)
LO-AOX1-His (Lpp-OmpA-Alcohol Oxidase)

metalP
BBa_K1342005 Version 1 (Component)
zinTp (Cd2+ sensing promoter) (Fw:Lac promoter(-) ver.)

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K1991008
BBa_K1991008 Version 1 (Component)
Pcons-RBS-LO-AOX1-His

BBa_K1991009
BBa_K1991009 Version 1 (Component)
Pcons-RBS-LO-AOX2-His

BBa_K315005
BBa_K315005 Version 1 (Component)
variant reverse lox site with 3 base changes in the spacer region (loxm2 reverse)

loxBri (R)
BBa_K315006 Version 1 (Component)
LoxBri (R)variant reverse lox site with 3 base changes in the spacer region

BBa_K185053
BBa_K185053 Version 1 (Component)
Promoter116+RBS34+LacI+Double terminator+Plac+RBS31+RelE+RBS30+Lon protease+Double terminator

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

BBa_K1091002
BBa_K1091002 Version 1 (Component)
green fluorescent protein－slow

BBa_S03736
BBa_S03736 Version 1 (Component)
pLac-lox-RBS-Tet (in pSB1A2)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.