BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

p(tetR)
BBa_R0040 Version 1 (Component)
TetR repressible promoter

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

lacZ-alpha
BBa_I732006 Version 1 (Component)
lacZ alpha fragment

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_K1847014
BBa_K1847014 Version 1 (Component)
placUV5-lacO

BBa_J33207
BBa_J33207 Version 1 (Component)
lac promoter and lacZ

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

BBa_K1675016
BBa_K1675016 Version 1 (Component)
P-atp2 mutant 140-B0034-lacz alpha

lacI
BBa_Q04121 Version 1 (Component)
QPI (B0034.C0012.B0015.R0011)

LacZ
BBa_I732005 Version 1 (Component)
lacZ (encoding beta-galactosidase, full-length)

BBa_K1675019
BBa_K1675019 Version 1 (Component)
P-atp2 mutant 202-B0034-lacz alpha

lacZ a
BBa_E0033 Version 1 (Component)
LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)

LacI
BBa_I732100 Version 1 (Component)
LacI

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

Cat
BBa_I14033 Version 1 (Component)
P(Cat)

BBa_P10599
BBa_P10599 Version 1 (Component)
GoldenBraid destination vector alpha insert

BBa_K1675021
BBa_K1675021 Version 1 (Component)
P-atp2 mutant 705-B0034-lacz alpha

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

BBa_K1675017
BBa_K1675017 Version 1 (Component)
P-atp2 mutant 430-B0034-lacz alpha

BBa_K1675018
BBa_K1675018 Version 1 (Component)
P-atp2 mutant 154-B0034-lacz alpha

tetR
BBa_Q04400 Version 1 (Component)
QPI (B0034.C0040.B0015.R0040)

BBa_K823003
BBa_K823003 Version 1 (Component)
P_veg

RhI
BBa_I14017 Version 1 (Component)
P(Rhl)

BBa_I732092
BBa_I732092 Version 1 (Component)
Double Repoters (LacZ-alpha and RFP)

BBa_Q04510
BBa_Q04510 Version 1 (Component)
QPI (B0034.C0051.B0015.R0051)

BBa_B0035
BBa_B0035 Version 1 (Component)
RBS (B0030 derivative)

BBa_K2066527
BBa_K2066527 Version 1 (Component)
B0034 with scar TACTAG

EYFP
BBa_E0034 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K208010
BBa_K208010 Version 1 (Component)
Lac Promoter (BBa_R0010) and RBS (B0034)

LacY
BBa_K137001 Version 1 (Component)
LacY

Las CIO
BBa_I14016 Version 1 (Component)
P(Las) CIO

BBa_B0024
BBa_B0024 Version 1 (Component)
double terminator (B0012-B0011), reversed

rhlR
BBa_C0071 Version 1 (Component)
rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)

BBa_K314103
BBa_K314103 Version 1 (Component)
Lac induced expression cassette

BBa_S0100
BBa_S0100 Version 1 (Component)
B0034.C0012

BBa_E0422
BBa_E0422 Version 1 (Component)
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)

rhIR
BBa_C0171 Version 1 (Component)
rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)

BBa_P0412
BBa_P0412 Version 1 (Component)
PoPS -> LacI [S0150]