BBa_K782028
BBa_K782028 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782029
BBa_K782029 Version 1 (Component)
10x[TALA] operator_minimal promoter_mCitrine

Bxb1_Xis
BBa_K907001 Version 1 (Component)
Mycobacterium Phage Bxb1 gp47, DNA excisionase

BBa_K782030
BBa_K782030 Version 1 (Component)
10x[TALB] operator_minimal promoter_mCitrine

BBa_K782031
BBa_K782031 Version 1 (Component)
12x[TALD] operator_minimal promoter_mCitrine

BBa_K782032
BBa_K782032 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_fLuciferase

BBa_K782033
BBa_K782033 Version 1 (Component)
10x[TALA] operator_minimal promoter_fLuciferase

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Cello E. Coli Parts
Eco1C1G1T1_collection Version 1 (Collection)
These are the Cello parts for E. Coli circuits

BBa_I730002
BBa_I730002 Version 1 (Component)
pLacI-tetR repressor

BBa_K098997
BBa_K098997 Version 1 (Component)
cIts coding region

BBa_K098998
BBa_K098998 Version 1 (Component)
cIts coding region with rbs

BBa_S04136
BBa_S04136 Version 1 (Component)
terminator and cI promoter

BBa_S04137
BBa_S04137 Version 1 (Component)
heat sensitive cI QPI

BBa_K098995
BBa_K098995 Version 1 (Component)
heat sensitive cI QPI with high promoter

BBa_K098993
BBa_K098993 Version 1 (Component)
heat sensitive cI QPI with low promoter

BBa_K098991
BBa_K098991 Version 1 (Component)
cI promoter with GFP reporter

BBa_K105021
BBa_K105021 Version 1 (Component)
cI - operator

BBa_S04138
BBa_S04138 Version 1 (Component)
cI QPI with GFP reporter system

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

Tm-Split-L
BBa_K157020 Version 1 (Component)
Tm-Split-Luciferase1/2

cI 434
BBa_R0052 Version 1 (Component)
Promoter (434 cI regulated)

Tm-Splitlu
BBa_K157021 Version 1 (Component)
Tm-Splitluciferase2/2

linker
BBa_K157013 Version 1 (Component)
15 aa flexible glycine-serine protein domain linker; Freiburg standard

SP-AntiNIP
BBa_K157030 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2

SP-AntiNIP
BBa_K157031 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2

SP-Lipocal
BBa_K157038 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2

SP-Lipocal
BBa_K157039 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2

Q04510+RFP
BBa_K131018 Version 1 (Component)
Intermediate for the Response circuit

BBa_K131019
BBa_K131019 Version 1 (Component)
LuxO inducible RFP

BBa_K131026
BBa_K131026 Version 1 (Component)
AHL inducible GFP

BBa_K090501
BBa_K090501 Version 1 (Component)
Gram-Positive IPTG-Inducible Promoter

BBa_K090502
BBa_K090502 Version 1 (Component)
Gram-Positive Xylose-Inducible Promoter

BBa_K123002
BBa_K123002 Version 1 (Component)
LacIQ ERE TetR

BBa_K082002
BBa_K082002 Version 1 (Component)
RBS defines RBS efficiency

BBa_K082004
BBa_K082004 Version 1 (Component)
LacI- wild type

BBa_K082005
BBa_K082005 Version 1 (Component)
LacI-Mutant

BBa_K090505
BBa_K090505 Version 1 (Component)
''Bacillus subtilis'' consensus RBS

BBa_K090506
BBa_K090506 Version 1 (Component)
''Bacillus subtilis'' weak RBS

BBa_K082012
BBa_K082012 Version 1 (Component)
B0034-LacI

BBa_K082034
BBa_K082034 Version 1 (Component)
lacI regulated GFP generator

BBa_K156011
BBa_K156011 Version 1 (Component)
aadA (streptomycin 3'-adenyltransferase)

BBa_K082028
BBa_K082028 Version 1 (Component)
R0040-B0034-LacI