BBa_K1124104
BBa_K1124104 Version 1 (Component)
clock device (plux/tet) (-luxI, -tetR, -aiiA, --sRNA, +amilGFP)(AHL sensor)

BBa_K2050422
BBa_K2050422 Version 1 (Component)
Mouse H1 promoter (RNA Polymerase III promoter)

BBa_K1363604
BBa_K1363604 Version 1 (Component)
key of no of RNA logic gates

BBa_K737050
BBa_K737050 Version 1 (Component)
The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42

BBa_K1124102
BBa_K1124102 Version 1 (Component)
clock device (plux/tet) (+luxI, -tetR, -aiiA, --sRNA, +amilGFP)(positive feedback only)

BBa_K563010
BBa_K563010 Version 1 (Component)
Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway

BBa_K1178000
BBa_K1178000 Version 1 (Component)
tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon

BBa_K1124103
BBa_K1124103 Version 1 (Component)
clock device (plux/tet) (-luxI, +tetR, +aiiA, ++sRNA, +amilGFP)(negative feedback only)

BBa_K077041
BBa_K077041 Version 1 (Component)
AiiA and cII under control of plac promotor

BBa_K1179017
BBa_K1179017 Version 1 (Component)
A mutant DNA binding Cas9 with an N-terminal linker and BsaI site for ligation

BBa_K1179008
BBa_K1179008 Version 1 (Component)
A mutant DNA binding Cas9 with a C-terminal linker and BsaI site for ligation

BBa_K896599
BBa_K896599 Version 1 (Component)
Strophurus rankini voucher AMS R140490 NADH dehydrogenase subunit 4 (ND4) gene, partial cds; tRNA-Hi

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

iGEM 2019 Plates
iGEM_2019_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2019

iGEM 2018 Plates
iGEM_2018_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2018

BBa_K1124100
BBa_K1124100 Version 1 (Component)
clock device (plux/tet) (+luxI, +tetR, +aiiA, ++sRNA, +amilGFP, +UV reset device(E234K, -LVA)

BBa_K880001
BBa_K880001 Version 1 (Component)
Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K

BBa_K323151
BBa_K323151 Version 1 (Component)
DNA program 123456

BBa_K323152
BBa_K323152 Version 1 (Component)
DNA Program 12346

BBa_K801032
BBa_K801032 Version 1 (Component)
GAL4 DNA binding domain

BBa_K105101
BBa_K105101 Version 1 (Component)
Gal4 - DNA binding domain

BBa_K105100
BBa_K105100 Version 1 (Component)
LacI - DNA binding domain

BBa_K887011
BBa_K887011 Version 1 (Component)
DNA program : specific DNA sequence which can be recognized by zinc fingers

BBa_K199091
BBa_K199091 Version 1 (Component)
pLac-CUACC Suppressor tRNA

BBa_J119088
BBa_J119088 Version 1 (Component)
pT7-RBS-GFP-tRNA (K191)

BBa_J119087
BBa_J119087 Version 1 (Component)
pT7-RBS-GFP-tRNA (K191)

CmsRNA
BBa_K1960102 Version 1 (Component)
sRNA, which can silence chloramphenicol resistance

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K199170
BBa_K199170 Version 1 (Component)
T7 Promoter RBS RFP pLac CCACC (tRNA)

BBa_K199169
BBa_K199169 Version 1 (Component)
T7 Promoter RBS RFP pLac CUAGC (tRNA)

BBa_K187254
BBa_K187254 Version 1 (Component)
folD, ORF, forward primer

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K809710
BBa_K809710 Version 1 (Component)
GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator

PchiP-lacZ
BBa_K564002 Version 1 (Component)
Chitoporin fused with lacZ - target for sRNA based regulation

BBa_K199070
BBa_K199070 Version 1 (Component)
I13453:K199016 Pbad promotor with the suppressor tRNA of CUAGC

BBa_K1942000
BBa_K1942000 Version 1 (Component)
A shRNA corresponding DNA sequence for KRAS which could silence the gene

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K199071
BBa_K199071 Version 1 (Component)
I13453:K199014: Pbad promomtor with the suppressor tRNA of the codon AGGAC

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.