BBa_K1675025BBa_K1675025 Version 1 (Component)P-atp2 mutant 399-B0034-lacz alpha
BBa_K1675024BBa_K1675024 Version 1 (Component)P-atp2 mutant 389-B0034-lacz alpha
BBa_K1675020BBa_K1675020 Version 1 (Component)P-atp2 mutant 226-B0034-lacz alpha
BBa_I715065BBa_I715065 Version 1 (Component)Burnt Pancake Tester with RE and backwards Tet resistance
BBa_K1195012BBa_K1195012 Version 1 (Component)T7 Bacteriophage Minor Capsid Protein (Large Mutant 2)
BBa_K806003BBa_K806003 Version 1 (Component)SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins
BBa_K1676088BBa_K1676088 Version 1 (Component)Mutant 12 of LDH and wildtype LDP
BBa_K1676091BBa_K1676091 Version 1 (Component)Mutant 15 of LDH and wildtype LDP
BBa_K1676086BBa_K1676086 Version 1 (Component)Mutant 10 of LDH and wildtype LDP
BBa_K1676080BBa_K1676080 Version 1 (Component)Mutant 4 of LDH and wildtype LDP
BBa_J44008BBa_J44008 Version 1 (Component)pLac-RBS-HinLVA-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF
BBa_J44006BBa_J44006 Version 1 (Component)pLac-RBS-HinLVA-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE
BBa_J44007BBa_J44007 Version 1 (Component)pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF
BBa_J44005BBa_J44005 Version 1 (Component)pLac-RBS-Hin-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE
BBa_M50056BBa_M50056 Version 1 (Component)PETase double mutant I208V and R90A full plasmid information
BBa_K133039BBa_K133039 Version 1 (Component)T7-CF-UREB-RGD-Histop
med-ABO_2830 Version 1 (Component) BBa_I715069BBa_I715069 Version 1 (Component)Test part to see if RE and pLac +hixc can be expressed with GFP
BBa_I758600BBa_I758600 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_K133037BBa_K133037 Version 1 (Component)T7-CF213-UB33-CF215-RGD-Histop
BBa_K346025BBa_K346025 Version 1 (Component)PmerT promoter mutant 88+RBS(B0030)+GFP(E0040)Terminator(B0010)+Terminator(B0012)
BBa_S03641BBa_S03641 Version 1 (Component)HixC : TetB-RBS<sub>rev</sub>-HixC
BBa_K133020BBa_K133020 Version 1 (Component)CMV-CF213-multiHP-CF215-RGD-Histop (term.)
BBa_S03638BBa_S03638 Version 1 (Component)HixC-pBad<sub>rev</sub>-HixC : RBS-TetF
BBa_M11085BBa_M11085 Version 1 (Component)E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o
BBa_K133023BBa_K133023 Version 1 (Component)CMV-ss-CF213-multiHP-CF215-RGD-Histop (term)
BBa_S03674BBa_S03674 Version 1 (Component)(-1,2) HixC-pBad<sub>rev</sub>-HixC : RBS-TetF-HixC
BBa_J22121BBa_J22121 Version 1 (Component)Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.