BBa_J24822
BBa_J24822 Version 1 (Component)
Same as J24819 but with the error at the luc-terminator junction fixed

BBa_K638201
BBa_K638201 Version 1 (Component)
Arabinose inducible Poly-His Reflectin A1 generator

BBa_K1403001
BBa_K1403001 Version 1 (Component)
GFP generator in pSB1C3 under a weak promoter

BBa_K855006
BBa_K855006 Version 1 (Component)
pvdQ gene with a silent mutation at 1494 bp to remove the internal PstI site

BBa_K855005
BBa_K855005 Version 1 (Component)
pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site

BBa_K299800
BBa_K299800 Version 1 (Component)
standard biobrick scar (if next part starts with ATG)

PL GFP
BBa_K193000 Version 1 (Component)
GFP reporter regulated by CI.

BBa_J119408
BBa_J119408 Version 1 (Component)
Pupp promoter mutant - Substitution of C and G to A at 28 and 30

BBa_K202004
BBa_K202004 Version 1 (Component)
Hybrid promoter having multiple operator sites. Promoter has tetO2 with mutation at position 3

BBa_K584023
BBa_K584023 Version 1 (Component)
Ice Neucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

BBa_I769103
BBa_I769103 Version 1 (Component)
Feruloyl-CoA hydratase for vanillin biosynthesis protein generator

BBa_K584022
BBa_K584022 Version 1 (Component)
Ice Neucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

BBa_K1088059
BBa_K1088059 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1088052
BBa_K1088052 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1178000
BBa_K1178000 Version 1 (Component)
tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon

pTetR-LacI
BBa_I763030 Version 1 (Component)
LacI coding device regulated by pTetR

BBa_K204040
BBa_K204040 Version 1 (Component)
C₄HSL generater

IodoY RS
BBa_K1416001 Version 1 (Component)
The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon

BBa_K511905
BBa_K511905 Version 1 (Component)
Repressible rtTA3 Transactivator Generator (Hef1a-LacO-rtTA3) MammoBlock Device

BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive

BBa_K1363201
BBa_K1363201 Version 1 (Component)
Anti-LPS factor(LALF) regulated by IPTG

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_K204022
BBa_K204022 Version 1 (Component)
3O₆HSL generater device

BBa_I763003
BBa_I763003 Version 1 (Component)
GFP coding device switched on by IPTG

RFC 37
BBa_K245088 Version 1 (Component)
TC-P5

BBa_K2172009
BBa_K2172009 Version 1 (Component)
Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin

BBa_K2123111
BBa_K2123111 Version 1 (Component)
Tac promoter with downstream mer operator + RFP (K081014)

BBa_K1615108
BBa_K1615108 Version 1 (Component)
mRFP fused to CBDclos driven by LacI promoter

PLac-LacY-
BBa_I763013 Version 1 (Component)
LacY and cI coding device switched on by IPTG

BBa_K258003
BBa_K258003 Version 1 (Component)
Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability

BBa_K395102
BBa_K395102 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)

BBa_K395103
BBa_K395103 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)

BBa_J58014
BBa_J58014 Version 1 (Component)
Promoter activated by OmpR-P with the reporter GFP

BBa_M36556
BBa_M36556 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

BBa_J58015
BBa_J58015 Version 1 (Component)
Mutated promoter activated by OmpR-P with the reporter GFP

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_K137021
BBa_K137021 Version 1 (Component)
GFP with (AC)20 repeat after start codon

BBa_K300096
BBa_K300096 Version 1 (Component)
Double phasin and intein separed by a flexible protein domain linker

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K2123117
BBa_K2123117 Version 1 (Component)
Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato

BBa_K2123115
BBa_K2123115 Version 1 (Component)
Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

PrtDEF
BBa_K258007 Version 1 (Component)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi