BBa_I741008
BBa_I741008 Version 1 (Component)
brings xylose into the cell (doesn

BBa_K145303
BBa_K145303 Version 1 (Component)
GFP generator controlled by key/lock

HE Promote
BBa_K1539055 Version 1 (Component)
HE Promoter Primer

HE RBS
BBa_K1539021 Version 1 (Component)
HE RBS Primer

BBa_J100198
BBa_J100198 Version 1 (Component)
J100198 contains the fitness gene tester construct (J100199) with thymidylate synthase as the fitnes

BBa_J64715
BBa_J64715 Version 1 (Component)
Constituive expression of LasR

BBa_J64716
BBa_J64716 Version 1 (Component)
Constituive expression of RHLR

BBa_K1638030
BBa_K1638030 Version 1 (Component)
Leucine zipper fused to T25 domain of <i>cyaA</i> with cAMP-induced RFP generator

BBa_K1638031
BBa_K1638031 Version 1 (Component)
Leucine zipper fused to T18 domain of <i>cyaA</i> with cAMP-induced RFP generator

BBa_I20275
BBa_I20275 Version 1 (Component)
LacI protein generator

BBa_I20272
BBa_I20272 Version 1 (Component)
LacI protein generator

YFP_SPtom2
BBa_K809318 Version 1 (Component)
YFP + signal peptide of Tom22

BBa_K150012
BBa_K150012 Version 1 (Component)
colicinE9 operon (short)

BBa_K1202109
BBa_K1202109 Version 1 (Component)
LsrK Generator

BBa_K511817
BBa_K511817 Version 1 (Component)
Inducible Yellow Fluorescent Protein Generator (TRE-Tight-EYFP-FF4x4) MammoBlock Device

BBa_K511906
BBa_K511906 Version 1 (Component)
Inducible CI434-VP16 Transactivator Generator (TRE-Tight-CI434-VP16) MammoBlock Device

BBa_K511910
BBa_K511910 Version 1 (Component)
Inducible LacI-KRAB Repressor Generator (TRE-Tight-LacI-KRAB) MammoBlock Device

BBa_K511908
BBa_K511908 Version 1 (Component)
Inducible Gal4-VP16 Transactivator Generator (TRE-Tight-Gal4-VP16) MammoBlock Device

BBa_K511814
BBa_K511814 Version 1 (Component)
Inducible Red Fluorescent Ligand Generator (TRE-Tight-Delta-mCherry) MammoBlock Device

BBa_K511907
BBa_K511907 Version 1 (Component)
Inducible Mnt-VP16 Transactivator Generator (TRE-Tight-Mnt-VP16) MammoBlock Device

Linker-FOS
BBa_K1616015 Version 1 (Component)
Linker FOS of 17 aa

Linker-JUN
BBa_K1616018 Version 1 (Component)
Linker JUN of 5 aa

BBa_I723126
BBa_I723126 Version 1 (Component)
This is the short description

BBa_K758000
BBa_K758000 Version 1 (Component)
UAS , this is the enhancer . And this enhancer is used to identify the protein localization .

BBa_K2004001
BBa_K2004001 Version 1 (Component)
Construct of CDO

BBa_K1461211
BBa_K1461211 Version 1 (Component)
arabinose-induced ecf11 generator

BBa_K1461209
BBa_K1461209 Version 1 (Component)
arabinose-induced ecf20 generator

BBa_I751500
BBa_I751500 Version 1 (Component)
pcI (for positive control of pcI-lux hybrid promoter)

BBa_K117009
BBa_K117009 Version 1 (Component)
colicin E7 production system induced by lactose

BBa_K2144002
BBa_K2144002 Version 1 (Component)
ESP capable of inhibiting biofilm

BBa_J58121
BBa_J58121 Version 1 (Component)
Detector of OmpR-P concentrations

BBa_J11013
BBa_J11013 Version 1 (Component)
tet regulated control of CI(ts)

BBa_J06650
BBa_J06650 Version 1 (Component)
Test of BBa_R0051

T18 domain
BBa_K1638004 Version 1 (Component)
T18 domain of CyaA from Bordetella pertussis

BBa_K594015
BBa_K594015 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces lasI and EYFP

CBPa-BpA
BBa_K2053001 Version 1 (Component)
Fusion cellulose-binding domain of CBP-B domain of protein A

BBa_K1937007
BBa_K1937007 Version 1 (Component)
AF-A : operon of antifungal peptides

BBa_K1365111
BBa_K1365111 Version 1 (Component)
dspB with signal sequence of USP45

BBa_K374002
BBa_K374002 Version 1 (Component)
the Gifsy-2 phage lysogenic repressor gtgR

BBa_K374000
BBa_K374000 Version 1 (Component)
the Gifsy-1 phage lysogenic repressor gogR

YFP_SPtom4
BBa_K809316 Version 1 (Component)
YFP + signal peptide of Tom40

BBa_K1660005
BBa_K1660005 Version 1 (Component)
RFP controlled by the PompR promoter

BBa_J23080
BBa_J23080 Version 1 (Component)
b0034 derived RBS to match spacing of J01122

BBa_K1614005
BBa_K1614005 Version 1 (Component)
RFC 110 forward fragment extraction primer

BBa_K1614006
BBa_K1614006 Version 1 (Component)
RFC 110 reverse fragment extraction primer

BBa_K1182401
BBa_K1182401 Version 1 (Component)
[Forward] Split &#946;-galactosidase - &#969; fragment

BBa_K1182501
BBa_K1182501 Version 1 (Component)
[Reverse] Split &#946;-galactosidase - &#969; fragment

BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_I2034
BBa_I2034 Version 1 (Component)
LacI protein generator

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.