BBa_K592032
BBa_K592032 Version 1 (Component)
J23101-B0032-YFP

YFP
BBa_K592101 Version 1 (Component)
Yellow Fluorescent Protein (YFP)

BBa_K542004
BBa_K542004 Version 1 (Component)
pLacI Regulated Lumazine Synthase with Transciptional Terminator and pBAD

BBa_K542005
BBa_K542005 Version 1 (Component)
pTet Regulated Arg-tagged ECFP and EYFP (FRET Reporter)

BBa_K542006
BBa_K542006 Version 1 (Component)
pBAD Inverse-Regulated Arg-tagged ECFP and EYFP (FRET Reporter)

BBa_K542008
BBa_K542008 Version 1 (Component)
pLacI Regulated Lumazine Synthase and pBAD Inverse-Regulated Arg-tagged ECFP and EYFP

BBa_K511101
BBa_K511101 Version 1 (Component)
EYFP-FF4x4 MammoBlock

BBa_K542010
BBa_K542010 Version 1 (Component)
Enhanced Lumazine Synthase (ELS)

BBa_K581008
BBa_K581008 Version 1 (Component)
RBS+ccdB+Termintor

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Tm-Fluo-li
BBa_K157014 Version 1 (Component)
Tm-Fluo-linker-cYFP

BBa_K157007
BBa_K157007 Version 1 (Component)
Split-Venus-cYFP

BBa_K157008
BBa_K157008 Version 1 (Component)
Split-Venus-nYFP

Tm-nYFP
BBa_K157015 Version 1 (Component)
Tm-nYFP

SP-AntiNIP
BBa_K157026 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP

SP-AntiNIP
BBa_K157027 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-nYFP

SP-Lipocal
BBa_K157034 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cYFP

SP-Lipocal
BBa_K157035 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-nYFP

BBa_K086000
BBa_K086000 Version 1 (Component)
unmodified Lutz-Bujard LacO promoter - YFP

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K112245
BBa_K112245 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid

BBa_K112246
BBa_K112246 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid

BBa_K112247
BBa_K112247 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112950 plasmid

BBa_K112248
BBa_K112248 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid

BBa_K086004
BBa_K086004 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP promoter

BBa_K086005
BBa_K086005 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086006
BBa_K086006 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086007
BBa_K086007 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP reporter

BBa_K086008
BBa_K086008 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086009
BBa_K086009 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP

BBa_K086010
BBa_K086010 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086011
BBa_K086011 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086012
BBa_K086012 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086013
BBa_K086013 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086016
BBa_K086016 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086014
BBa_K086014 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086015
BBa_K086015 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K146001
BBa_K146001 Version 1 (Component)
AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002

BBa_K165058
BBa_K165058 Version 1 (Component)
Kozak + YFP

BBa_K076004
BBa_K076004 Version 1 (Component)
EYFP

TNY2AKUB
BBa_K076000 Version 1 (Component)
pLV-TRE-Ngn1-linker-EYFP-2A-mKate-Ubc-Bla

BBa_K076007
BBa_K076007 Version 1 (Component)
pLV-TRE-Ngn1-linker-EYFP-Ubc-Bla

BBa_Z0301
BBa_Z0301 Version 1 (Component)
Venus YFP - E. coli codon optimized

eyfp
BBa_E0031 Version 1 (Component)
EYFP (RBS+ LVA-)

BBa_J70311
BBa_J70311 Version 1 (Component)
R0040 (TetR promotor), E0031 (RBS+EYFP)

BBa_J70312
BBa_J70312 Version 1 (Component)
E0031 (RBS+EYFP), B0014 (terminator)

BBa_J70316
BBa_J70316 Version 1 (Component)
Calibration of YFP input to RFP (mcherry) output

BBa_J70317
BBa_J70317 Version 1 (Component)
Calibration of RFP (mcherry) input to YFP output