BBa_K323088
BBa_K323088 Version 1 (Component)
In vivo testing device for protein-DNA binding: part 1 (DTER_pSYN_BbsI)

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

BBa_K2170117
BBa_K2170117 Version 1 (Component)
Intermediate of secretion efficiency construct (NanoLuc_StrepTag_PolyA) in RFC[25] C-part

BBa_M31338
BBa_M31338 Version 1 (Component)
Part of g2 gene from HpaI site to end of gene

BBa_J70609
BBa_J70609 Version 1 (Component)
RFC12 7x His Tag Head Domain

BBa_M30110
BBa_M30110 Version 1 (Component)
part of the M13 viral genome for annotation/refactoring

BBa_K112995
BBa_K112995 Version 1 (Component)
BBb1 assembly vector - C/A

BBa_K594011
BBa_K594011 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.

BBa_K1707028
BBa_K1707028 Version 1 (Component)
CFP-ssRA downstream of a cI regulated promoter

BBa_K2170215
BBa_K2170215 Version 1 (Component)
Eukaryotic signal peptide IgKappa (from immunoglobuline Kappa chain komplex) in RFC[25] N-part

BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

BBa_K078003
BBa_K078003 Version 1 (Component)
one part of the initial dioxygenase of the dioxin degradation pathway

BBa_K1159013
BBa_K1159013 Version 1 (Component)
Secretory Human Protein Phosphatase 1 (SERK-SigP_PP1) in RFC[25] N-Part

BBa_K1159009
BBa_K1159009 Version 1 (Component)
Secretory Erythromycin Esterase Type II (SERK-SigP_EreB) in RFC[25] N-Part

BBa_K801040
BBa_K801040 Version 1 (Component)
SV40NLS-PhyB-linker-Gal4DBD (part for GAL4 based light switchable promoter system)

BBa_J70664
BBa_J70664 Version 1 (Component)
Test assembly for Grant's parts

BBa_K1159017
BBa_K1159017 Version 1 (Component)
Membrane-anchored FluA triple mutant in RFC[25] N-Part

BBa_K1669000
BBa_K1669000 Version 1 (Component)
CtfA + His-tag

BBa_K2066037
BBa_K2066037 Version 1 (Component)
pACT-Tet on UNS Standard

BBa_K1497005
BBa_K1497005 Version 1 (Component)
B0034-CHS-B0034-CHI - Composite part of RBS-CHS and RBS-CHI

BBa_K2170116
BBa_K2170116 Version 1 (Component)
Intermediate of biotin binding receptor (A3C5_EGFR TMD_mRuby3_StrepTag_PolyA) in RFC[25] C-part

BBa_K323005
BBa_K323005 Version 1 (Component)
CMV ATG PBSII link nYFP stop - part of mammalian split/FRET system

BBa_K2170115
BBa_K2170115 Version 1 (Component)
Intermediate of biotin binding receptor (EGFR TMD_mRuby3_StrepTag_PolyA) in RFC[25] C-part

BBa_K323021
BBa_K323021 Version 1 (Component)
CMV ATG Gli1 link nCFP stop - part of mammalian split/FRET system

BBa_K1159312
BBa_K1159312 Version 1 (Component)
N-terminal <i>Strep</i>-tag-II in RFC[25] N-Part

BBa_K2170144
BBa_K2170144 Version 1 (Component)
Intermediate of biotin binding receptor (scAvidin_A3C5_EspP_double terminator) in RFC[25] C-part

BBa_K2170122
BBa_K2170122 Version 1 (Component)
Intermediate of biotin binding receptor (EGFR TMD_mRuby3_StrepTag) in RFC[25] C-part

BBa_K2170123
BBa_K2170123 Version 1 (Component)
Intermediate of signal peptide testing construct (mRuby3_StrepTag) in RFC[25] N-part

BBa_K2170124
BBa_K2170124 Version 1 (Component)
Intermediate of signal peptide testing construct (mRuby3_StrepTag_polyA) in RFC[25] N-part

BBa_K2170142
BBa_K2170142 Version 1 (Component)
Intermediate of biotin binding receptor (NanoLuc_StrepTag_double terminator) in RFC[25] C-part

BBa_J70342
BBa_J70342 Version 1 (Component)
J70315psri.f.1: J70315 psri forward part for pcr of S03621 (single stranded)

BBa_K2170143
BBa_K2170143 Version 1 (Component)
Intermediate of biotin binding receptor (BAP_NanoLuc_A3C5_EspP_double terminator) in RFC[25] C-part

BBa_J70343
BBa_J70343 Version 1 (Component)
J70315psri.r.1: J70315 psri reverse part for pcr of S03621 (single stranded)

BBa_K2170221
BBa_K2170221 Version 1 (Component)
OmpA signal peptide with tetracycline operator (TetO_OmpA) in RFC[25] N-part

VR
BBa_G00101 Version 1 (Component)
Reverse primer for sequencing/amplifying BioBrick parts (VR)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K223003
BBa_K223003 Version 1 (Component)
Terminator plus SoxS promoter- part to be appended to SoxR gene

BBa_K1159111
BBa_K1159111 Version 1 (Component)
Membrane-associated Thermonuclease NucA with TEV site and SV40 NLS in RFC[25] N-Part

Fla-Art175
BBa_K1659001 Version 1 (Component)
Artilysin Art-175 fused at N-terminal with flagellin 26-47 peptide segment

BBa_K180019
BBa_K180019 Version 1 (Component)
Scissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI generator]

BBa_K180015
BBa_K180015 Version 1 (Component)
Paper - Primary plasmid (part 2) [tac pR, GFP and RhlI generator]

BBa_K180012
BBa_K180012 Version 1 (Component)
Rock - Primary plasmid (part 2) [tac pR, mCherry and LasI generator]

BBa_K1523007
BBa_K1523007 Version 1 (Component)
A Device to reduce Cr(VI)

BBa_J70589
BBa_J70589 Version 1 (Component)
N-terminal protein tag addition test part J70576:S04431(J70575:E0040)

BBa_K1159014
BBa_K1159014 Version 1 (Component)
Membrane-anchored Erythromycin Esterase Type II in RFC[25] N-Part

BBa_K1159008
BBa_K1159008 Version 1 (Component)
Secretory Catechol-2,3-dioxygenase (IgKappa-SigP_XylE) in RFC[25] N-Part

BBa_K1159018
BBa_K1159018 Version 1 (Component)
Membrane-anchored Catechol-2,3-dioxygenase (XylE) in RFC[25] N-Part

BBa_J119142
BBa_J119142 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

BBa_K235022
BBa_K235022 Version 1 (Component)
[K235018][K235019] (mCherry generator, pAra-controlled ribokey-mediated signal inversion)

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin