BBa_J133421
BBa_J133421 Version 1 (Component)
Translational Stop Sequence 1:001

BBa_K608151
BBa_K608151 Version 1 (Component)
translational unit of pcyA

BBa_K916004
BBa_K916004 Version 1 (Component)
Leucine Zipper CGFP translational fusion

BBa_K916003
BBa_K916003 Version 1 (Component)
Leucine Zipper NGFP translational fusion

BBa_K1635000
BBa_K1635000 Version 1 (Component)
BBa_K1635000 (Archaeal translational efficiency part)

BBa_K1631005
BBa_K1631005 Version 1 (Component)
Translational unit of Barstar (Barnase immunity protein)

BBa_K1523102
BBa_K1523102 Version 1 (Component)
The translational unit of yieF

BBa_K173013
BBa_K173013 Version 1 (Component)
pdc translational unit (RBS B0030)

BBa_K173014
BBa_K173014 Version 1 (Component)
adhB translational unit (RBS B0030)

BBa_K1300010
BBa_K1300010 Version 1 (Component)
cI repressible promoter + translational ccdB

BBa_K1114704
BBa_K1114704 Version 1 (Component)
BioBrick adaption of MoClo Level 1 RFP Reporter

BBa_K1178001
BBa_K1178001 Version 1 (Component)
Plasmid Backbone pSB1C95 for RFC 95 Open Sequence Initiative

BBa_K175042
BBa_K175042 Version 1 (Component)
GFP-LVA translational unit + I-SceI RS + TetR generator

BBa_K1523103
BBa_K1523103 Version 1 (Component)
The translational unit of yieF

BBa_K1058002
BBa_K1058002 Version 1 (Component)
Translational unit of penicillinase repressor blaI

BBa_K1819002
BBa_K1819002 Version 1 (Component)
Twin-arginine translocation signal (TAT signal)

BBa_K806002
BBa_K806002 Version 1 (Component)
hda - regulator of DnaA that prevents premature reinitiation of DNA replication

BBa_K1758102
BBa_K1758102 Version 1 (Component)
Translation enhancing 5-UTR + sfGFP under control of T7 promoter

BBa_K1223007
BBa_K1223007 Version 1 (Component)
cI translational unit with His-tag

BBa_K2182003
BBa_K2182003 Version 1 (Component)
Translational Unit for His-tagged NOX

BBa_K1223011
BBa_K1223011 Version 1 (Component)
ampR translational unit (ampicilin resistance CDS+promoter)

BBa_I20257
BBa_I20257 Version 1 (Component)
Autoregulated synthesis of T7 RNAP with lacI negative feedback (orthogonal translation)

BBa_K1963006
BBa_K1963006 Version 1 (Component)
A translational fusion between E. coli OsmY and Serratia Hfq

LacIm
BBa_K092800 Version 1 (Component)
Coding sequence for LacI modified with a different rate of translation than LacI, RBS

BBa_K1100151
BBa_K1100151 Version 1 (Component)
AAC(6')-Ib translational unit with B0015 terminator

BBa_K801030
BBa_K801030 Version 1 (Component)
Polypeptide containing SV40 nuclear localization sequence (SV40 NLS) for nuclear translocation

BBa_K415204
BBa_K415204 Version 1 (Component)
B0034 : cymR Repressor Protein (Strong cymR Translational Unit)

BBa_K1963005
BBa_K1963005 Version 1 (Component)
A translational fusion between E. coli OsmY and E. coli Hfq

BBa_K1903003
BBa_K1903003 Version 1 (Component)
Golden Gate Adapter

BBa_K1159117
BBa_K1159117 Version 1 (Component)
Red light triggered Kill Switch for plants translation unit (PhyB/PIF3 version)

BBa_K1159118
BBa_K1159118 Version 1 (Component)
Red light triggered Kill Switch for plants translation unit (PhyB/PIF6 version)

BBa_K1613012
BBa_K1613012 Version 1 (Component)
QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.

BBa_K1159114
BBa_K1159114 Version 1 (Component)
Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF6 based)

BBa_K1159113
BBa_K1159113 Version 1 (Component)
Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF3 based)

BBa_K563004
BBa_K563004 Version 1 (Component)
pTEF1, promoter of Translational elongation factor EF-1 alpha

BBa_K1465222
BBa_K1465222 Version 1 (Component)
Carboxysome (shell-proteins (csoS14)) with translational GFP fusion (under T7 and without csoS2)

Adapter Bi
BBa_K1807015 Version 1 (Component)
This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of

BBa_K1159116
BBa_K1159116 Version 1 (Component)
Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF6 based) with downstream IRES

BBa_K1159115
BBa_K1159115 Version 1 (Component)
Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF3 based) with downstream IRES

BBa_K1159120
BBa_K1159120 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)

BBa_K1159119
BBa_K1159119 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF3 version)

BBa_K1159214
BBa_K1159214 Version 1 (Component)
Membrane-anchored SpyTag with secretory nLuc_SpyCatcher reporter fusion translational unit

BBa_K1159213
BBa_K1159213 Version 1 (Component)
Membrane-anchored SpyCatcher with secretory nLuc_SpyTag reporter fusion translational unit

BBa_K1159212
BBa_K1159212 Version 1 (Component)
Membrane-anchored SpyCatcher with secretory SpyTag_nLuc reporter fusion translational unit

BBa_K1159215
BBa_K1159215 Version 1 (Component)
Membrane-anchored SpyTag with secretory SpyCatcher_nLuc reporter fusion translational unit

BBa_K327013
BBa_K327013 Version 1 (Component)
The initiator

BBa_K1162014
BBa_K1162014 Version 1 (Component)
protmoter+rbs+N-terminal 10x His-Tag+GFP with no translational stop codon

BBa_T6002
BBa_T6002 Version 1 (Component)
test translational unit

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.