BBa_K157002
BBa_K157002 Version 1 (Component)
Transmembrane region of the EGF-Receptor (ErbB-1)

BBa_K113021
BBa_K113021 Version 1 (Component)
Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_K098999
BBa_K098999 Version 1 (Component)
mtrB with RBS

BBa_K098998
BBa_K098998 Version 1 (Component)
cIts coding region with rbs

BBa_K098996
BBa_K098996 Version 1 (Component)
mtrB with terminator

BBa_K098994
BBa_K098994 Version 1 (Component)
mtrB with RBS and terminator

BBa_K098995
BBa_K098995 Version 1 (Component)
heat sensitive cI QPI with high promoter

BBa_K098993
BBa_K098993 Version 1 (Component)
heat sensitive cI QPI with low promoter

BBa_K098991
BBa_K098991 Version 1 (Component)
cI promoter with GFP reporter

BBa_S04138
BBa_S04138 Version 1 (Component)
cI QPI with GFP reporter system

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

pRecA
BBa_K093000 Version 1 (Component)
pRecA with LexA binding site

BBa_J24815
BBa_J24815 Version 1 (Component)
J23101 promoter with Renilla gene attached

BBa_J24816
BBa_J24816 Version 1 (Component)
J23101 promoter with Luc gene attached

linker
BBa_K157013 Version 1 (Component)
15 aa flexible glycine-serine protein domain linker; Freiburg standard

BBa_K143067
BBa_K143067 Version 1 (Component)
AmyE integratable PoPS generator (P43-gsiB)

BBa_K143068
BBa_K143068 Version 1 (Component)
AmyE integratable PoPS generator (P43-spoVG)

BBa_K143069
BBa_K143069 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-gsiB)

Eukaryotic
BBa_K157040 Version 1 (Component)
Eukaryotic terminator vector (very similar to pSB1AK8)

Transfecti
BBa_K157041 Version 1 (Component)
Transfection vector (similar to pSB1AK8)

BBa_K102951
BBa_K102951 Version 1 (Component)
TA1In anti-sense input to TA1 (BBa_K102901)

BBa_K102952
BBa_K102952 Version 1 (Component)
TA2In anti-sense input to BBa_K102952

BBa_K102953
BBa_K102953 Version 1 (Component)
TA13n anti-sense input to TA3 (BBa_K102903)

BBa_K102954
BBa_K102954 Version 1 (Component)
TA6In anti-sense input to BBa_K102904

BBa_K102955
BBa_K102955 Version 1 (Component)
TA7In anti-sense input to BBa_K102905

BBa_K102956
BBa_K102956 Version 1 (Component)
TA8In anti-sense input to BBa_K102906

BBa_K102957
BBa_K102957 Version 1 (Component)
TA9In anti-sense input to BBa_K102907

BBa_K102958
BBa_K102958 Version 1 (Component)
TA10In anti-sense input to BBa_K102908

BBa_K102959
BBa_K102959 Version 1 (Component)
TA11In anti-sense input to BBa_K102909

BBa_K102960
BBa_K102960 Version 1 (Component)
TA12In anti-sense input to anti-terminator BBa_K102910

BBa_K102961
BBa_K102961 Version 1 (Component)
TA13In anti-sense input to BBa_K102911

BBa_K102962
BBa_K102962 Version 1 (Component)
TA14In anti-sense input to BBa_K102912

BBa_K143070
BBa_K143070 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-spoVG)

His
BBa_K157011 Version 1 (Component)
His affinity tag; Freiburg standard

BBa_K157012
BBa_K157012 Version 1 (Component)
Strep affinity tag II; Freiburg standard

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_K143071
BBa_K143071 Version 1 (Component)
AmyE integratable PoPS generator (P43-gsiB) (with CmR)

BBa_K143072
BBa_K143072 Version 1 (Component)
AmyE integratable PoPS generator (P43-spoVG) (with CmR)

BBa_K143073
BBa_K143073 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)

BBa_K143074
BBa_K143074 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)

BBa_J69522
BBa_J69522 Version 1 (Component)
tetR-repressible promoter coupled with mCherry Reporter

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP