BBa_K774003
BBa_K774003 Version 1 (Component)
Comparator Circuit Part 2

BBa_K769003
BBa_K769003 Version 1 (Component)
Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator

BBa_K759006
BBa_K759006 Version 1 (Component)
B0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_K914002
BBa_K914002 Version 1 (Component)
repressilator RBS Colicin E2 Immunity protein

BBa_J72218
BBa_J72218 Version 1 (Component)
kanamycin resistant p15A vector

BBa_K782016
BBa_K782016 Version 1 (Component)
10x[TALA+TALB] operator_CMV promoter_mCitrine

BBa_K782017
BBa_K782017 Version 1 (Component)
10x[TALA] operator_CMV promoter_mCitrine

BBa_K782018
BBa_K782018 Version 1 (Component)
10x[TALB] operator_CMV promoter_mCitrine

BBa_K782019
BBa_K782019 Version 1 (Component)
10x[TALA+TALB] operator_CMV promoter_fLuciferase

BBa_K782020
BBa_K782020 Version 1 (Component)
10x[TALA] operator_CMV promoter_fLuciferase

BBa_K782021
BBa_K782021 Version 1 (Component)
10x[TALB] operator_CMV promoter_fLuciferase

BBa_K782022
BBa_K782022 Version 1 (Component)
12x[TALD] operator_CMV promoter_fLuciferase

gpS
BBa_K836004 Version 1 (Component)
Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)

BBa_K782023
BBa_K782023 Version 1 (Component)
12x[NicTAL] operator_CMV promoter_fLuciferase

BBa_K782024
BBa_K782024 Version 1 (Component)
2x[NicTAL]+2x[TALD] operator_minimal promoter_mCitrine

BBa_K782025
BBa_K782025 Version 1 (Component)
4x[NicTAL]+4x[TALD] operator_minimal promoter_mCitrine

BBa_K782026
BBa_K782026 Version 1 (Component)
7x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782027
BBa_K782027 Version 1 (Component)
7x[TALA] operator_minimal promoter_mCitrine

BBa_K782028
BBa_K782028 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782029
BBa_K782029 Version 1 (Component)
10x[TALA] operator_minimal promoter_mCitrine

sboA-AmilC
BBa_K818400 Version 1 (Component)
AmilCP with sboA regulator

BBa_K818500
BBa_K818500 Version 1 (Component)
Lycopene with sboA regulator

sboA-AmilG
BBa_K818600 Version 1 (Component)
AmilGFP with sboA regulator

BBa_K863020
BBa_K863020 Version 1 (Component)
bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag

BM-CFP
BBa_K774004 Version 1 (Component)
Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG promoter sequence + BBa_E0420

BM-RFP
BBa_K774005 Version 1 (Component)
Bacterial-Mammalian promoter with RFP reporter: BBaK216005 + BHN + CArG promoter sequence E9-ns2 + B

MB-CFP
BBa_K774006 Version 1 (Component)
Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420

MB-RFP
BBa_K774007 Version 1 (Component)
Mammalian-Bacterial promoter with RFP reporter: CArG promoter sequence + BBaK216005 + BBa_K08101

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K759007
BBa_K759007 Version 1 (Component)
RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

mtGFP
BBa_K809001 Version 1 (Component)
Mitochondrial GFP

BBa_K782030
BBa_K782030 Version 1 (Component)
10x[TALB] operator_minimal promoter_mCitrine

BBa_K782031
BBa_K782031 Version 1 (Component)
12x[TALD] operator_minimal promoter_mCitrine

nitR
BBa_K836007 Version 1 (Component)
Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)

BBa_K782032
BBa_K782032 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_fLuciferase

BBa_K782033
BBa_K782033 Version 1 (Component)
10x[TALA] operator_minimal promoter_fLuciferase

BBa_K1982002
BBa_K1982002 Version 1 (Component)
Prokaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K113004
BBa_K113004 Version 1 (Component)
pT7->tetR operator->GFP

BBa_S04136
BBa_S04136 Version 1 (Component)
terminator and cI promoter

BBa_K113005
BBa_K113005 Version 1 (Component)
pLac->tetR->pT7->tetR operator ->GFP

BBa_K105020
BBa_K105020 Version 1 (Component)
tetR - operator

BBa_K105021
BBa_K105021 Version 1 (Component)
cI - operator

BBa_K105022
BBa_K105022 Version 1 (Component)
lex A - operator

BBa_K105023
BBa_K105023 Version 1 (Component)
lac I - operator

BBa_K105024
BBa_K105024 Version 1 (Component)
Gal4 - operator

BBa_K105025
BBa_K105025 Version 1 (Component)
Adh1 terminator