BBa_K187216
BBa_K187216 Version 1 (Component)
rnc, ORF, forward primer

BBa_K187271
BBa_K187271 Version 1 (Component)
rne ORF, Forward Primer

BBa_K2050423
BBa_K2050423 Version 1 (Component)
tRNA(Val) and linker

BBa_K1223013
BBa_K1223013 Version 1 (Component)
Pyrolysyl-tRNA synthetase CDS

BBa_K1741002
BBa_K1741002 Version 1 (Component)
sfGFP under a short arabinose promoter s2 without O1 and O2

BBa_K1189001
BBa_K1189001 Version 1 (Component)
TALE-B with a his 6 tag under a lacI promoter

pBAD-AB-DT
BBa_K323135 Version 1 (Component)
VioA and VioB enzymes fused with zinc fingers under pBAD promoter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene

BBa_K563003
BBa_K563003 Version 1 (Component)
promoter of CIT2

BisdAB
BBa_K525517 Version 1 (Component)
Fusion protein of BisdA and BisdB behind constitutive promoter

BBa_J24804
BBa_J24804 Version 1 (Component)
This is Renilla Luciferase under constitutive promoter with two terminators.

BBa_K1631002
BBa_K1631002 Version 1 (Component)
Translational unit of Colicin-E3

BBa_M36658
BBa_M36658 Version 1 (Component)
mRNA Sequence for Acetoacetyl-CoA Reductase

BBa_K784048
BBa_K784048 Version 1 (Component)
T7*(K1F) RNAP double stop codon

BBa_K784052
BBa_K784052 Version 1 (Component)
T7*(T3) RNAP double stop codon

BBa_J100125
BBa_J100125 Version 1 (Component)
This oligo is the promoter sequence for the dnaQ gene.

BBa_J34900
BBa_J34900 Version 1 (Component)
the best RBS ever!

BBa_J119056
BBa_J119056 Version 1 (Component)
pLacI-RBS-T7 RNAP (2SAT mutant 2)

BBa_J119059
BBa_J119059 Version 1 (Component)
pLacI-RBS-T7 RNAP (2SAT mutant 5)

BBa_K1083003
BBa_K1083003 Version 1 (Component)
The HEWL antigen generator

BBa_M31000
BBa_M31000 Version 1 (Component)
refactoring the M13 genome

BBa_K152011
BBa_K152011 Version 1 (Component)
GFP RNAi construct

RFP-BA-Bbs
BBa_K349003 Version 1 (Component)
RFP-BA-BbsI (for construction of BA BioBytes 2.0)

BBa_K2066536
BBa_K2066536 Version 1 (Component)
Spinach Aptamer w/ tRNA Scaffold

BBa_K2113002
BBa_K2113002 Version 1 (Component)
temperature sensitive system with lambda pr promoter under cI regulation

BBa_K1631000
BBa_K1631000 Version 1 (Component)
Translational unit of Barnase

BBa_K1123017
BBa_K1123017 Version 1 (Component)
DNA Coding Sequence for EGFP Protein

BBa_K874040
BBa_K874040 Version 1 (Component)
125bp DNA spacer (inc. preceding BamHI site)

BBa_K874041
BBa_K874041 Version 1 (Component)
125bp DNA spacer (inc. trailing NdeI site)

Bm3R1
BBa_K1401000 Version 1 (Component)
TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')

Devices from the iGEM 2016 interlab
iGEM_2016_interlab_collection Version 1 (Collection)
This is a collection of devices that were used in the 2016 iGEM interlab study

BBa_K119002
BBa_K119002 Version 1 (Component)
RcnR operator (represses RcnA)

BBa_I738010
BBa_I738010 Version 1 (Component)
The parts for plasmid 2 for bacteria 2 for the warefare

BBa_I738009
BBa_I738009 Version 1 (Component)
The parts for plasmid 1 for bacteria 1 for the warefare

BBa_K1159307
BBa_K1159307 Version 1 (Component)
35S Terminator of Cauliflower Mosaic Virus (CaMV)

BBa_K1351006
BBa_K1351006 Version 1 (Component)
CWB domain of B. subtilis major autolysin LytC

BBa_K1207000
BBa_K1207000 Version 1 (Component)
Gram positive origin of replication

BBa_K809301
BBa_K809301 Version 1 (Component)
RFP + signal peptide of ZIM17

BBa_K105003
BBa_K105003 Version 1 (Component)
tetR - DNA binding domain

BBa_I766002
BBa_I766002 Version 1 (Component)
YopJ, Irrev. MAPKK Ser/Thr Acetylase

E coil fer
BBa_K1189037 Version 1 (Component)
His tag + Human ferritin di-subunit with E coil under LacI promoter

BBa_K1175200
BBa_K1175200 Version 1 (Component)
the whole magilua

BBa_K2039005
BBa_K2039005 Version 1 (Component)
Coding sequences of protein GFP 1.9 (subunit of tripartite GFP)

BBa_K199156
BBa_K199156 Version 1 (Component)
pLac-RBS-CCACU-RFP-pLpp-CUAGU tRNA-CCACU tRNA

BBa_K567002
BBa_K567002 Version 1 (Component)
sulA promoter-tRNA(Arg)

BBa_K1179048
BBa_K1179048 Version 1 (Component)
U6-gRNA 6A Reverse

BBa_K1017403
BBa_K1017403 Version 1 (Component)
sRNA-1 (with Plux)

BBa_K323214
BBa_K323214 Version 1 (Component)
TAL (DNA binding protein)