BBa_K1728018
BBa_K1728018 Version 1 (Component)
DUSP1 toehold switch RNA sensor with T7 promoter & luciferase reporter

BBa_K1728016
BBa_K1728016 Version 1 (Component)
IL8 toehold switch RNA sensor with T7 promoter & luciferase reporter

BBa_K1728019
BBa_K1728019 Version 1 (Component)
SAT toehold switch RNA sensor with T7 promoter & luciferase reporter

BBa_K165008
BBa_K165008 Version 1 (Component)
YY1 DNA-binding domain

BBa_C100008
BBa_C100008 Version 1 (Component)
ORF of EEB1 odorant enzyme

BBa_J120020
BBa_J120020 Version 1 (Component)
CH1 domain of human antibody

BBa_K734002
BBa_K734002 Version 1 (Component)
Spinach Aptamer with Stabilizing tRNA Scaffold

BBa_K1124112
BBa_K1124112 Version 1 (Component)
sRNA scaffold (-antisense, +terminator)

BBa_K1450001
BBa_K1450001 Version 1 (Component)
T7 RNA polymerase

BBa_I737000
BBa_I737000 Version 1 (Component)
from the workshop

BBa_K1795109
BBa_K1795109 Version 1 (Component)
J23117 N20 gRNA sequence

BBa_K1795105
BBa_K1795105 Version 1 (Component)
R0011 N20 gRNA Sequence

BBa_K1795112
BBa_K1795112 Version 1 (Component)
I0500 N20 gRNA sequence

BBa_K1795103
BBa_K1795103 Version 1 (Component)
R0051 gRNA N20 Sequence

BBa_K1795108
BBa_K1795108 Version 1 (Component)
J23106 N20 gRNA Sequence

BBa_K1795111
BBa_K1795111 Version 1 (Component)
I13453 N20 gRNA Sequence

BBa_K1607011
BBa_K1607011 Version 1 (Component)
The SCFV of anti-p185her2/neu antibody chA21 linked with EGFP by a (Gly4Ser)3 linker

BBa_I1040
BBa_I1040 Version 1 (Component)
RNA Regulation Target ( Promoter driven by C0010 and cI(2) CDS)

BBa_K1758377
BBa_K1758377 Version 1 (Component)
Biosensor device for detection of GHB and GBL

BBa_K1132013
BBa_K1132013 Version 1 (Component)
heme oxygenase (ho1), ferredoxin oxidoreductase (PcyA) and tetracycline repressor (TetR) under const

BBa_K784050
BBa_K784050 Version 1 (Component)
T7* RNAP double stop codon

BBa_M1026
BBa_M1026 Version 1 (Component)
DNA Scaffold for Binding Domains

BBa_K575006
BBa_K575006 Version 1 (Component)
Continous expression of RhlR receptor protein(with RBS, B0030)

BBa_K1983018
BBa_K1983018 Version 1 (Component)
PheP under constitutive promoter, low strength RBS and terminator

BBa_K1983017
BBa_K1983017 Version 1 (Component)
PheP under constitutive promoter, medium strength RBS and terminator

TetR+tfa+
BBa_K1846007 Version 1 (Component)
Bacteriophage lambda tail fibre assembly (tfa) under TetR regulation

BBa_K1983014
BBa_K1983014 Version 1 (Component)
PheP under constitutive promoter, high strength RBS and terminator

BBa_K105005
BBa_K105005 Version 1 (Component)
lexA - DNA binding domain

BBa_K105006
BBa_K105006 Version 1 (Component)
lacI - DNA binding domain

BBa_K1431503
BBa_K1431503 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431501
BBa_K1431501 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431502
BBa_K1431502 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K327014
BBa_K327014 Version 1 (Component)
The initiator

BBa_K1861020
BBa_K1861020 Version 1 (Component)
Fusion protein of the TALE constant domain and a SpyTag in an expression cassette

BBa_K1878001
BBa_K1878001 Version 1 (Component)
iRFP713 (the fluorescent protein formerly known as "iRFP")

BBa_K1189014
BBa_K1189014 Version 1 (Component)
GFP fused to an E coil under a lacI promoter

BBa_K1741013
BBa_K1741013 Version 1 (Component)
sfGFP under proC promoter with a hairpin in 5'UTR

BBa_K1189000
BBa_K1189000 Version 1 (Component)
TALE-A with a his tag under a lacI promoter

BBa_K1189017
BBa_K1189017 Version 1 (Component)
K coil and 6X His Tag under inducible LacI promoter

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K784044
BBa_K784044 Version 1 (Component)
T7*(N4) RNAP double stop codon

pyrG casse
BBa_K678040 Version 1 (Component)
pyrG cassette, for selection of Aspergillus nidulans

BBa_K1129042
BBa_K1129042 Version 1 (Component)
4-coumarate-CoA ligase (4CL) under pTET constitutive promoter

BBa_K1114516
BBa_K1114516 Version 1 (Component)
Level 1 MoClo Device that expresses tetR under pBAD

BBa_K1114511
BBa_K1114511 Version 1 (Component)
Level 1 MoClo device that expresses YFP under pBAD

BBa_K1129026
BBa_K1129026 Version 1 (Component)
Phenylalanine Ammonia Lyase (Enc-P) under pTET constitutive promoter

BBa_K1114510
BBa_K1114510 Version 1 (Component)
Level 1 MoClo device that expresses YFP under pTetR

BBa_K1129022
BBa_K1129022 Version 1 (Component)
Enoyl-CoA hydratase/aldolase (Ech) under pTET constitutive promoter

BBa_K1179045
BBa_K1179045 Version 1 (Component)
U6-gRNA 4A

BBa_K1835000
BBa_K1835000 Version 1 (Component)
Empty gRNA Scaffold