BBa_S01830BBa_S01830 Version 1 (Component)Intermediate part from assembly 240
BBa_S01845BBa_S01845 Version 1 (Component)Intermediate part from assembly 240
BBa_S01380BBa_S01380 Version 1 (Component)Intermediate part from assembly 239
BBa_S01377BBa_S01377 Version 1 (Component)Intermediate part from assembly 239
BBa_S01842BBa_S01842 Version 1 (Component)Intermediate part from assembly 240
Q04510+RFPBBa_K131018 Version 1 (Component)Intermediate for the Response circuit
BBa_S01383BBa_S01383 Version 1 (Component)Intermediate part from assembly 239
BBa_S01843BBa_S01843 Version 1 (Component)Intermediate part from assembly 240
BBa_S01378BBa_S01378 Version 1 (Component)Intermediate part from assembly 239
BBa_S01826BBa_S01826 Version 1 (Component)Intermediate part from assembly 240
BBa_S01382BBa_S01382 Version 1 (Component)Intermediate part from assembly 239
BBa_S01821BBa_S01821 Version 1 (Component)Intermediate part from assembly 240
BBa_K1983016BBa_K1983016 Version 1 (Component)PheP under constitutive promoter and tRNA-Phe under DH10B promoter
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_S02001BBa_S02001 Version 1 (Component)Intermediate part from assembly 240
LacYBBa_K079014 Version 1 (Component)LacY transporter protein from E. coli
BBa_K1072016BBa_K1072016 Version 1 (Component)pGAL1 + Brho + EGFP + ADH1 Te
BBa_K1351017BBa_K1351017 Version 1 (Component)SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>
BBa_K619894BBa_K619894 Version 1 (Component)mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K1447004BBa_K1447004 Version 1 (Component)Epitope 1-5 from Ara h 1
BBa_K1447005BBa_K1447005 Version 1 (Component)Epitope 1-5 from Ara h 1
BBa_K619895BBa_K619895 Version 1 (Component)mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K833002BBa_K833002 Version 1 (Component)mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K833006BBa_K833006 Version 1 (Component)Mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K833008BBa_K833008 Version 1 (Component)Mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K1072013BBa_K1072013 Version 1 (Component)pGAL1+ Flag + odr-10 + GFP + ADH1 Te
BBa_K511817BBa_K511817 Version 1 (Component)Inducible Yellow Fluorescent Protein Generator (TRE-Tight-EYFP-FF4x4) MammoBlock Device
BBa_K511906BBa_K511906 Version 1 (Component)Inducible CI434-VP16 Transactivator Generator (TRE-Tight-CI434-VP16) MammoBlock Device
BBa_K511910BBa_K511910 Version 1 (Component)Inducible LacI-KRAB Repressor Generator (TRE-Tight-LacI-KRAB) MammoBlock Device
BBa_K511908BBa_K511908 Version 1 (Component)Inducible Gal4-VP16 Transactivator Generator (TRE-Tight-Gal4-VP16) MammoBlock Device
BBa_K511814BBa_K511814 Version 1 (Component)Inducible Red Fluorescent Ligand Generator (TRE-Tight-Delta-mCherry) MammoBlock Device
BBa_K511907BBa_K511907 Version 1 (Component)Inducible Mnt-VP16 Transactivator Generator (TRE-Tight-Mnt-VP16) MammoBlock Device
BBa_K1114003BBa_K1114003 Version 1 (Component)The MoClo format of BBa_J23103 with AB fusion sites.
BBa_K2066500BBa_K2066500 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
BBa_M36914BBa_M36914 Version 1 (Component)Codes for the benzylalcohol acetyltransferase enzyme
BBa_K1441013BBa_K1441013 Version 1 (Component)DNA ligase from Escherichia coli with His-tag INSERT
BBa_K1766008BBa_K1766008 Version 1 (Component)EnvZ_V5 osmoregulatory histidine kinase from <i>E.coli</i>.
BBa_K1195004BBa_K1195004 Version 1 (Component)Cro from Lamda phage with shine delgarno added.
BBa_K1766014BBa_K1766014 Version 1 (Component)EnvZ osmoregulatory histidine kinase from <i>E.coli.</i>
BBa_K1072014BBa_K1072014 Version 1 (Component)pGAL1+ Flag + Brho + odr-10 + GFP + ADH1 Te
BBa_K1431101BBa_K1431101 Version 1 (Component)TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA
BBa_K1947017BBa_K1947017 Version 1 (Component)We verify the expression effect of Mms13.
BBa_K1497197BBa_K1497197 Version 1 (Component)B0034-CHI - Chalcone Isomerase from Petunia with strong RBS
BBa_K1465203BBa_K1465203 Version 1 (Component)Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Synechococcus elongatus
pLacIQ1-RBBBa_K193404 Version 1 (Component)coding a set of enzymes (ho1 and pcyA) producing PCB from heam
BBa_K511818BBa_K511818 Version 1 (Component)Inducible Notch-Gal4-ESN Receptor Generator (TRE-Tight-Notch-Gal4-ESN) MammoBlock Device
BBa_K1051262BBa_K1051262 Version 1 (Component)The measurement pathway of degradation tag K1051208.