Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K1847014
BBa_K1847014 Version 1 (Component)
placUV5-lacO

lacI
BBa_Q04121 Version 1 (Component)
QPI (B0034.C0012.B0015.R0011)

BBa_K142004
BBa_K142004 Version 1 (Component)
lacI IS mutant (IPTG unresponsive) R197A T276A

LacI
BBa_I732100 Version 1 (Component)
LacI

BBa_K142006
BBa_K142006 Version 1 (Component)
lacI IS mutant (IPTG unresponsive) R197F T276A

BBa_K142005
BBa_K142005 Version 1 (Component)
lacI IS mutant (IPTG unresponsive) R197A T276F

BBa_K142007
BBa_K142007 Version 1 (Component)
lacI IS mutant (IPTG unresponsive) R197F T276F

lacZ-alpha
BBa_I732006 Version 1 (Component)
lacZ alpha fragment

BBa_J33207
BBa_J33207 Version 1 (Component)
lac promoter and lacZ

BBa_K142002
BBa_K142002 Version 1 (Component)
lacI IS mutant (IPTG unresponsive) T276A

BBa_K142000
BBa_K142000 Version 1 (Component)
lacI IS mutant (IPTG unresponsive) R197A

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

BBa_K142003
BBa_K142003 Version 1 (Component)
lacI IS mutant (IPTG unresponsive) T276F

BBa_K142001
BBa_K142001 Version 1 (Component)
lacI IS mutant (IPTG unresponsive) R197F

BBa_P0412
BBa_P0412 Version 1 (Component)
PoPS -> LacI [S0150]

BBa_K142020
BBa_K142020 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276A) generator

BBa_K142022
BBa_K142022 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276A) generator

BBa_K142021
BBa_K142021 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276F) generator

LacZ
BBa_I732005 Version 1 (Component)
lacZ (encoding beta-galactosidase, full-length)

LacY
BBa_K137001 Version 1 (Component)
LacY

BBa_K142023
BBa_K142023 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276F) generator

BBa_K142012
BBa_K142012 Version 1 (Component)
lacI IS mutant R197A T276A with RBS and terminator

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

BBa_K731500
BBa_K731500 Version 1 (Component)
[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward

BBa_K314103
BBa_K314103 Version 1 (Component)
Lac induced expression cassette

BBa_K142014
BBa_K142014 Version 1 (Component)
lacI IS mutant R197F T276A with RBS and terminator

BBa_K142013
BBa_K142013 Version 1 (Component)
lacI IS mutant R197A T276F with RBS and terminator

BBa_K082005
BBa_K082005 Version 1 (Component)
LacI-Mutant

LacI
BBa_K143033 Version 1 (Component)
LacI (Lva^-, N-terminal deletion) regulatory protein

BBa_K142015
BBa_K142015 Version 1 (Component)
lacI IS mutant R197F T276F with RBS and terminator

BBa_K142018
BBa_K142018 Version 1 (Component)
tet-controlled lacI IS mutant (T276A) generator

BBa_K142016
BBa_K142016 Version 1 (Component)
tet-controlled lacI IS mutant (R197A) generator

BBa_K142028
BBa_K142028 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette

BBa_K864400
BBa_K864400 Version 1 (Component)
Ptac, trp & lac regulated promoter

lasI
BBa_C0178 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)