BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_K2097000
BBa_K2097000 Version 1 (Component)
CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.

BBa_K2082231
BBa_K2082231 Version 1 (Component)
RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434

pLacIQ+eGF
BBa_K193207 Version 1 (Component)
TmTHP with promotor for constituitive expression and with EGFP for fusion of Anti-Freeze Protein

T7+eGFP+Tm
BBa_K193208 Version 1 (Component)
TmTHP with promotor for constituitive expression and with EGFP for fusion of Anti-Freeze Protein

BBa_K1861020
BBa_K1861020 Version 1 (Component)
Fusion protein of the TALE constant domain and a SpyTag in an expression cassette

BBa_K2150027
BBa_K2150027 Version 1 (Component)
pCon+RBS+tetR+DT+pT7+pTet+RBS+tetX-GFP(fusion protein)+RBS+T7RNAP+DT

PcotV
BBa_K823033 Version 1 (Component)
P_<i>cotV</i>: <i>B. subtilis</i> promoter regulates spore crust protein cotV

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

BBa_K258016
BBa_K258016 Version 1 (Component)
Vhb protein

BBa_K2082237
BBa_K2082237 Version 1 (Component)
RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein

BBa_K861160
BBa_K861160 Version 1 (Component)
CRP, cAMP receptor protein

BBa_K079049
BBa_K079049 Version 1 (Component)
GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator

BBa_K079020
BBa_K079020 Version 1 (Component)
GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein

BBa_K1088009
BBa_K1088009 Version 1 (Component)
B. subtilis dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG ind

BBa_K1088010
BBa_K1088010 Version 1 (Component)
E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib

BBa_M1530
BBa_M1530 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP

BBa_M1515
BBa_M1515 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_M1529
BBa_M1529 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_M1541
BBa_M1541 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_M1531
BBa_M1531 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_M1542
BBa_M1542 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_M1535
BBa_M1535 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_K2150029
BBa_K2150029 Version 1 (Component)
pCon+RBS+tetR+DT+pTet+RBS+T7RNAP+Ter+RBS+pT7+tetX-GFP(fusion protein)+DT

BBa_M1536
BBa_M1536 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP

BBa_K563010
BBa_K563010 Version 1 (Component)
Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway

BBa_K2088006
BBa_K2088006 Version 1 (Component)
It encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. I

BBa_K431002
BBa_K431002 Version 1 (Component)
Rattus norvegicus monoamine oxidase A (Maoa), nuclear gene encoding mitochondrial protei

BBa_K1051116
BBa_K1051116 Version 1 (Component)
Actin Targeting Protein

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K987000
BBa_K987000 Version 1 (Component)
This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p

enhanced
BBa_K2075011 Version 1 (Component)
enhanced Green fluorescenc protein (GFP)

enhanced G
BBa_K2075012 Version 1 (Component)
enhanced Green fluorescenc protein (GFP)

BBa_J58009
BBa_J58009 Version 1 (Component)
Fusion protein Trg-EnvZ

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_K1123017
BBa_K1123017 Version 1 (Component)
DNA Coding Sequence for EGFP Protein

BBa_K1014016
BBa_K1014016 Version 1 (Component)
HrpS Protein Generator

BBa_I721004
BBa_I721004 Version 1 (Component)
Lead Binding Protein + Promoter (version a)