BBa_K112217BBa_K112217 Version 1 (Component){a~ihfA!} The ihf alpha gene ready to have rbs attached with stop codon, BBb format
BBa_K112223BBa_K112223 Version 1 (Component){a~ihfB!} The ihf beta gene ready to have rbs attached and stop codon, BBb format
BBa_K112211BBa_K112211 Version 1 (Component){a~int!} The integrase gene with rbs ready to be attached with stop codon, BBb format
BBa_K2066084BBa_K2066084 Version 1 (Component)Promoter characterization part - J23103 without RiboJ
BBa_K1655001BBa_K1655001 Version 1 (Component)This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.
BBa_K2066063BBa_K2066063 Version 1 (Component)Promoter characterization part: J23103 with RiboJ
BBa_K880001BBa_K880001 Version 1 (Component)Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K
AckABBa_K737002 Version 1 (Component)This coding is the acetate kinase.
BBa_K2176001BBa_K2176001 Version 1 (Component)Cassette for the constitutive production of GAL4-KaiCp and LexA-SasA, linked by a self-cleaving P2A
BBa_K1216014BBa_K1216014 Version 1 (Component)BBa_K1216014 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.
BBa_K1216012BBa_K1216012 Version 1 (Component)BBa_K1216012 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.
BBa_K1216013BBa_K1216013 Version 1 (Component)BBa_K1216013 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.
BBa_K1216011BBa_K1216011 Version 1 (Component)BBa_K1216007 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.
BBa_K1216010BBa_K1216010 Version 1 (Component)BBa_K1216010 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL. (
BBa_J14462BBa_J14462 Version 1 (Component)Composite part comprised of J13002 and J04650
xisBBa_K112204 Version 1 (Component){a~xis!} The bacteriophage lambda xis gene ready to have rbs attached and stop codon; assembly stand
BBa_Z0271BBa_Z0271 Version 1 (Component)T7.2 TE transcriptional terminator
BBa_J85900BBa_J85900 Version 1 (Component)TRE-tight-B1-LacI-T2A-EBFP2-FF5-miRFF4
BBa_K368024BBa_K368024 Version 1 (Component)tet-on Promoter (prev TRE) + attB (bacterial attachment site)
BBa_K1361004BBa_K1361004 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag
BBa_K2082237BBa_K2082237 Version 1 (Component)RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein
BBa_K1113501BBa_K1113501 Version 1 (Component)GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag
BBa_K079052BBa_K079052 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato
Co-opBBa_K233002 Version 1 (Component)Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway
BBa_K233001BBa_K233001 Version 1 (Component)Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway
BBa_K332024BBa_K332024 Version 1 (Component)A part of cell-cell-sigaling system
BBa_K079020BBa_K079020 Version 1 (Component)GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein
BBa_I745008BBa_I745008 Version 1 (Component)pLV-TH-siGFP
BBa_K080007BBa_K080007 Version 1 (Component)TetO (TRE)-nkx-2.5-fmdv2A-gata4-fmdv2A-dsRed
BBa_J14465BBa_J14465 Version 1 (Component)Composite part comprised of J13002 and I13401
BBa_J14459BBa_J14459 Version 1 (Component)Composite part comprised of J04500 and J04630
BBa_J14464BBa_J14464 Version 1 (Component)Composite part comprised of J13002 and J04630
BBa_K626000BBa_K626000 Version 1 (Component)TRE-Bax-PolyA in PSB1A3 (expression vector)
BBa_K327013BBa_K327013 Version 1 (Component)The initiator
BBa_K564016BBa_K564016 Version 1 (Component)Upstream mutated chitoporin part fused with lacZ
BBa_K564017BBa_K564017 Version 1 (Component)Upstream mutated chitoporin part fused with lacZ
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K175035BBa_K175035 Version 1 (Component)Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock
BBa_K175034BBa_K175034 Version 1 (Component)Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock
BBa_K076007BBa_K076007 Version 1 (Component)pLV-TRE-Ngn1-linker-EYFP-Ubc-Bla
BBa_K1113411BBa_K1113411 Version 1 (Component)Targeting sequence for the delivery of the LacZ gene to the Carboxysome
BBa_K1983015BBa_K1983015 Version 1 (Component)PheP and tRNA-Phe under constitutive promoters
BBa_K1116002BBa_K1116002 Version 1 (Component)TRE--LacI with T21 and miR-FF3 target
BBa_K080008BBa_K080008 Version 1 (Component)TetO (TRE)-nkx-2.5-fmdv2A-gata4-fmdv2A-dsRed
BBa_K1189029BBa_K1189029 Version 1 (Component)TALE-A with a his tag linked to a K coil under the control of a LacI promoter
BBa_K581003BBa_K581003 Version 1 (Component)SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)