BBa_I3420

BBa_I3420 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I3420
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Date created: 2004-01-28 12:00:00
Date modified: 2015-08-31 04:07:41

Test: Switch functionality and bistability (I3410.E0422.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3



VisBOL
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Truncate Labels
BBa_B0012BBa_B0010cI lamBBa_B0034AraCBBa_B0012BBa_B0010araCBBa_B0034const.BBa_B0012BBa_B0010cI HK022BBa_B0034p(tetR)BBa_B0012BBa_B0010tetRBBa_B0034const.BBa_B0012BBa_B0010cI HK022BBa_B0034LacIBBa_B0012BBa_B0010lacIBBa_B0034const.BBa_B0012BBa_B0010YFPBBa_B0034cI lamBBa_B0034cI HK022BBa_B0012BBa_B0010ECFPBBa_B0034cI HK022BBa_B0034cI lam
Types
DnaRegion

Roles
engineered_region

Project

Sequences BBa_I3420_sequence (Version 1)

Description

Tests input levels needed to trigger switch, bistability, noise resistance, and switch time. Switch has three independently controllable trigger inputs and two reporter outputs, ON and OFF.

Notes

The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000).

Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432).

The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I3420/1