Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Date created: 2004-01-28 12:00:00
Date modified: 2015-08-31 04:07:41
Test: Switch functionality and bistability (I3410.E0422.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_I3420_sequence (Version 1) |
Description
Tests input levels needed to trigger switch, bistability, noise resistance, and switch time. Switch has three independently controllable trigger inputs and two reporter outputs, ON and OFF.Notes
The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000).Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432).
The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).