BBa_I3421

BBa_I3421 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I3421
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Date created: 2004-01-28 12:00:00
Date modified: 2015-08-31 04:07:41

Test: Switch response to aspartate input (I3410.E0422.I3411.E0432.I3100.I3400.I3300.I3400.I3102.I340



Types
DnaRegion

Roles
engineered_region

Project

Sequences BBa_I3421_sequence (Version 1)

Description

Tests ability of switch to respond to aspartate input by connecting the Tar-EnvZ receptor (I3300 ompC upstream version) to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).

Notes

The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the other (I3300.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I3421/1