BBa_K1911007

BBa_K1911007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1911007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jackson Harris
Date created: 2016-10-20 11:00:00
Date modified: 2016-10-21 01:29:10

pLamdaR-LacI-GFP-pLac-ClpXP-CI



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1911007_sequence (Version 1)

Description

This construct is an example of the switch system that was used in our project. Once the E. coli cells containing our construct was induced with IPTG, the pLamdaR pathway would be shut down while the pLac pathway would be turned on. This allows for the degradation of the gene of interest in which this case is the eGFP. This construct is the finished product of our project after using 3A Assembly to assemble the two different promoter pathways. This construct is switchable because IPTG can be added to switch the system from one pathway to another. NOTE: There is no Biobrick scar between Bba_K1911005 and Bba_M0051. The Sequence given will contain a scar of, "tactagag" which is to be ignored.

Notes

No

Source

It is a composite part from multiple, individual Genomic sequences that have been assembled via AAA Assembly

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1911007/1