CBDcex(T7)BBa_K863102 Version 1 (Component)Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
BBa_K113015BBa_K113015 Version 1 (Component)bidirectional intact T7 promoters->GFP/RFP
BBa_K113016BBa_K113016 Version 1 (Component)overlapping T7 promoter->GFP/RFP
BBa_K113017BBa_K113017 Version 1 (Component)more overlapping T7 promoter->GFP/RFP
BBa_K113018BBa_K113018 Version 1 (Component)weaken overlapping T7 promoter->GFP/RFP
BBa_K113021BBa_K113021 Version 1 (Component)Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
BBa_K098999BBa_K098999 Version 1 (Component)mtrB with RBS
BBa_K098998BBa_K098998 Version 1 (Component)cIts coding region with rbs
BBa_K098996BBa_K098996 Version 1 (Component)mtrB with terminator
BBa_K098994BBa_K098994 Version 1 (Component)mtrB with RBS and terminator
BBa_K098995BBa_K098995 Version 1 (Component)heat sensitive cI QPI with high promoter
BBa_K098993BBa_K098993 Version 1 (Component)heat sensitive cI QPI with low promoter
BBa_K098991BBa_K098991 Version 1 (Component)cI promoter with GFP reporter
BBa_S04138BBa_S04138 Version 1 (Component)cI QPI with GFP reporter system
BBa_K098988BBa_K098988 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and high promoter
BBa_K098987BBa_K098987 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and low promoter
BBa_K098982BBa_K098982 Version 1 (Component)lac inducible system with low promoter and GFP reporter
pRecABBa_K093000 Version 1 (Component)pRecA with LexA binding site
BBa_J24815BBa_J24815 Version 1 (Component)J23101 promoter with Renilla gene attached
BBa_J24816BBa_J24816 Version 1 (Component)J23101 promoter with Luc gene attached
BBa_K131019BBa_K131019 Version 1 (Component)LuxO inducible RFP
mRFP1BBa_I13502 Version 1 (Component)Screening plasmid intermediate
Q04510+RFPBBa_K131018 Version 1 (Component)Intermediate for the Response circuit
BBa_K101016BBa_K101016 Version 1 (Component)Dually repressed promoter with sites for TetR and P22MNT binding
BBa_K143071BBa_K143071 Version 1 (Component)AmyE integratable PoPS generator (P43-gsiB) (with CmR)
BBa_K143072BBa_K143072 Version 1 (Component)AmyE integratable PoPS generator (P43-spoVG) (with CmR)
BBa_K143073BBa_K143073 Version 1 (Component)AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)
BBa_K143074BBa_K143074 Version 1 (Component)AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)
BBa_J69522BBa_J69522 Version 1 (Component)tetR-repressible promoter coupled with mCherry Reporter
BBa_K143076BBa_K143076 Version 1 (Component)B. subtilis AmyE locus RFP output promoter and RBS tester
BBa_K143080BBa_K143080 Version 1 (Component)P43-gsiB RFP expression construct
BBa_K143081BBa_K143081 Version 1 (Component)P43-spoVG RFP expression construct
BBa_K143082BBa_K143082 Version 1 (Component)Pveg-spoVG RFP expression construct
BBa_K122016BBa_K122016 Version 1 (Component)4CL:STS with CYC1 terminator.
RBS-RFPBBa_K093005 Version 1 (Component)RFP with RBS
RBS-cIBBa_K093006 Version 1 (Component)cI repressor with RBS
oriTr/LacZBBa_K125350 Version 1 (Component)oriTr with lac promoter and lacZ
oriT/LacZBBa_K125360 Version 1 (Component)oriT with lac promoter and lacZ
BBa_K086001BBa_K086001 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter
BBa_K086018BBa_K086018 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
BBa_K086002BBa_K086002 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP
BBa_K086019BBa_K086019 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
BBa_K086003BBa_K086003 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter
BBa_K086020BBa_K086020 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
BBa_K142008BBa_K142008 Version 1 (Component)lacI IS mutant R197A with RBS and terminator
BBa_K142009BBa_K142009 Version 1 (Component)lacI IS mutant R197F with RBS and terminator