BBa_K098991
BBa_K098991 Version 1 (Component)
cI promoter with GFP reporter

BBa_S04138
BBa_S04138 Version 1 (Component)
cI QPI with GFP reporter system

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

pRecA
BBa_K093000 Version 1 (Component)
pRecA with LexA binding site

BBa_J24815
BBa_J24815 Version 1 (Component)
J23101 promoter with Renilla gene attached

BBa_J24816
BBa_J24816 Version 1 (Component)
J23101 promoter with Luc gene attached

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K113015
BBa_K113015 Version 1 (Component)
bidirectional intact T7 promoters->GFP/RFP

BBa_K113021
BBa_K113021 Version 1 (Component)
Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_K143071
BBa_K143071 Version 1 (Component)
AmyE integratable PoPS generator (P43-gsiB) (with CmR)

BBa_K143072
BBa_K143072 Version 1 (Component)
AmyE integratable PoPS generator (P43-spoVG) (with CmR)

BBa_K143073
BBa_K143073 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)

BBa_K143074
BBa_K143074 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)

BBa_J69522
BBa_J69522 Version 1 (Component)
tetR-repressible promoter coupled with mCherry Reporter

BBa_K093011
BBa_K093011 Version 1 (Component)
reverse B0015

BBa_K122016
BBa_K122016 Version 1 (Component)
4CL:STS with CYC1 terminator.

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

RBS-cI
BBa_K093006 Version 1 (Component)
cI repressor with RBS

oriTr/LacZ
BBa_K125350 Version 1 (Component)
oriTr with lac promoter and lacZ

oriT/LacZ
BBa_K125360 Version 1 (Component)
oriT with lac promoter and lacZ

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086018
BBa_K086018 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP

BBa_K086019
BBa_K086019 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086020
BBa_K086020 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

rPlac+TT
BBa_K093004 Version 1 (Component)
Convergent Promoter System: Reverse Module: rPlac+TT

BBa_K093008
BBa_K093008 Version 1 (Component)
reverse BBa_R0011

BBa_K142008
BBa_K142008 Version 1 (Component)
lacI IS mutant R197A with RBS and terminator

BBa_K142009
BBa_K142009 Version 1 (Component)
lacI IS mutant R197F with RBS and terminator

BBa_K142010
BBa_K142010 Version 1 (Component)
lacI IS mutant T276A with RBS and terminator

BBa_K142011
BBa_K142011 Version 1 (Component)
lacI IS mutant T276Fwith RBS and terminator

BBa_K142012
BBa_K142012 Version 1 (Component)
lacI IS mutant R197A T276A with RBS and terminator

BBa_K142013
BBa_K142013 Version 1 (Component)
lacI IS mutant R197A T276F with RBS and terminator

BBa_K142014
BBa_K142014 Version 1 (Component)
lacI IS mutant R197F T276A with RBS and terminator

BBa_K142015
BBa_K142015 Version 1 (Component)
lacI IS mutant R197F T276F with RBS and terminator

BBa_K142202
BBa_K142202 Version 1 (Component)
SceI with RBS (BBa_B0030) and double terminator (BBa_B0015)

BBa_K142203
BBa_K142203 Version 1 (Component)
T4 ligase with RBS (BBa_B0030) and double terminator (BBa_B0015)

BBa_K106672
BBa_K106672 Version 1 (Component)
AarI AD acceptor vector (pRS305, Gal1P, Adh1t)

BBa_K106686
BBa_K106686 Version 1 (Component)
8xLexA Operators

BBa_K142047
BBa_K142047 Version 1 (Component)
tet-controlled LacI generator with constitutive TetR expression cassette

BBa_K142024
BBa_K142024 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette

BBa_K142025
BBa_K142025 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette

BBa_K142026
BBa_K142026 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette

BBa_K142027
BBa_K142027 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette