Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
ATGATG Version 1 (Component) Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
HisTagBBa_J18909 Version 1 (Component)His tag (6xHis; E. coli - optimized)
BBa_K1933011BBa_K1933011 Version 1 (Component)INPNC fused to 6xHis tag without stop codon
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
INP-HisBBa_K1933001 Version 1 (Component)INPNC fused to 6xHis tag
BBa_J04031BBa_J04031 Version 1 (Component)GFP w/LVA tag
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
HABBa_I712008 Version 1 (Component)HA affinity tag
NLSBBa_J176008 Version 1 (Component)NLS
BclA-HisBBa_K1933000 Version 1 (Component)N-terminal domain of BclA protein fused to 6xHis tag
BBa_K105012BBa_K105012 Version 1 (Component)10 aa flexible protein domain linker
PESTBBa_J176018 Version 1 (Component)PEST
BBa_K1933100BBa_K1933100 Version 1 (Component)constitutive expression of CBDcex fused to INPNC with 6xHis tag
BBa_K1933101BBa_K1933101 Version 1 (Component)constitutive expression of CBDclos fused to INPNC with 6xHis tag
linkerBBa_K133132 Version 1 (Component)8 aa protein domain linker
OmpABBa_K103006 Version 1 (Component)OmpA outer membrane protein A fused to linker; displays proteins on cell surface
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
NLSmycSTOPBBa_J176031 Version 1 (Component)NLS myc stop
RGD-His-StBBa_K133036 Version 1 (Component)RGD-His affinity tag-Stop
BBa_J44001BBa_J44001 Version 1 (Component)Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
BBa_K1933200BBa_K1933200 Version 1 (Component)constitutive expression of anti-Norovirus GII.4 scFv fused to INPNC with 6xHis tag
CD4 signalBBa_I712009 Version 1 (Component)CD4 signal peptide; localizes to plasma membrane
G-His6(N)BBa_K1362430 Version 1 (Component)C-terminal His6 (N-Intein)
BBa_J70042BBa_J70042 Version 1 (Component)6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)
BBa_K1933103BBa_K1933103 Version 1 (Component)constitutive expression of CBDclos fused to BclA with 6xHis tag
BBa_K1933102BBa_K1933102 Version 1 (Component)constitutive expression of CBDcex fused to BclA with 6xHis tag
BBa_K416003BBa_K416003 Version 1 (Component)Yeast Secretion Tag
RBS-GFP-TeBBa_I763020 Version 1 (Component)RBS-GFP (+LVA Tag)-Terminators
BBa_K1933003BBa_K1933003 Version 1 (Component)constitutive expression of CBDclos fused to BclA with 6xHis tag
BBa_K1321342BBa_K1321342 Version 1 (Component)sfGFP fused to CBDcex in RFC 25
polyABBa_J176009 Version 1 (Component)polyA2
BBa_K1321341BBa_K1321341 Version 1 (Component)sfGFP fused to CBDclos in RFC 25
BBa_K1321122BBa_K1321122 Version 1 (Component)Smt fused to CBDcex