GFP
GFP Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S

HYG
Hygromicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

NEO (G418/Neomicin Resistance)
Neomicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

mCherry
mCherry Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

amdS
amdS Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

APP1
GenBank_CP047908_Region_1244167_1244926 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)

ACT1
GenBank_CP003820_Region_1242235_1243067 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_B1001
BBa_B1001 Version 1 (Component)
Terminator (artifical, small, %T~=90)

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

RFC105 A
BBa_K1362440 Version 1 (Component)
N-terminal start overhang (T)(A)-GATG=RBS+Start RFC[105] A

Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_B1006
BBa_B1006 Version 1 (Component)
Terminator (artificial, large, %T~>90)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

RFC105 NN
BBa_K1362415 Version 1 (Component)
N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

BBa_K809601
BBa_K809601 Version 1 (Component)
GAL promoter (kozak) without start codon ATG

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

RFC105 CC
BBa_K1362419 Version 1 (Component)
C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

RFC 37
BBa_K245009 Version 1 (Component)
RFC 37 Scar Sequence

RFC105 CN
BBa_K1362418 Version 1 (Component)
N-terminal GG-linker overhang <u>GGTG</u>=Gly+1/3Gly RFC[105] overhang CN

BBa_K1321340
BBa_K1321340 Version 1 (Component)
Double CBD (dCBD) with N-terminal linker

T7start
BBa_J18912 Version 1 (Component)
T7 promoter + RBS + START