BBa_K2012009BBa_K2012009 Version 1 (Component)RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal
BBa_K730000BBa_K730000 Version 1 (Component)The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide.
BBa_K235025BBa_K235025 Version 1 (Component)[K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal)
BBa_J100015BBa_J100015 Version 1 (Component)1 Clause 2-SAT Problem with Frameshifted LuxI and a GFP Reporter
BBa_K382069BBa_K382069 Version 1 (Component)intron hairpin RNAi knockdown of Bet v 1 allergen in Kan plant vector
BBa_M36475BBa_M36475 Version 1 (Component)Codes for AraC, a transcription factor which represses operon. Arabinose dislodges TF protein.
BBa_S03251BBa_S03251 Version 1 (Component)Intermediate part from assembly 317
BBa_S01522BBa_S01522 Version 1 (Component)Intermediate part from assembly 240
TSensorBBa_J11021 Version 1 (Component)Tunable Temperature Sensor v2 (using LacI ts + other parts from Registry 7.05)
BBa_K1333310BBa_K1333310 Version 1 (Component)RFP with an RNA thermometer(FourU)
BBa_K1333311BBa_K1333311 Version 1 (Component)RFP with an RNA thermometer(PrfA)
BBa_K387007BBa_K387007 Version 1 (Component)OmpA: An excretory signal peptide in bacteria
TrfABBa_K112129 Version 1 (Component)TrfA with an RBS , followed by terminiator
BBa_K1412089BBa_K1412089 Version 1 (Component)Riboregulator which combines crRNA and RBS acting as a lock to the gene cuicuit
IBMc334IBMc334 Version 1 (Component)pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-FKBP-T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter
IBMc335IBMc335 Version 1 (Component)pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter
BBa_M36916BBa_M36916 Version 1 (Component)This series of parts codes for the expression of the benzylalcohol acetyltransferase enzyme
BBa_S01412BBa_S01412 Version 1 (Component)Intermediate part from assembly 240
BBa_K1419002BBa_K1419002 Version 1 (Component)RNA-OUT component for an Arabinose inducible lysis casette
BBa_J580110BBa_J580110 Version 1 (Component)This is my first part
BBa_K1172306BBa_K1172306 Version 1 (Component)Riboflavin synthesis gene cluster from s. oneidensis under control of a strong Anderson promoter
BBa_K1172305BBa_K1172305 Version 1 (Component)Riboflavin synthesis gene cluster from s. oneidensis under control of a medium Anderson promoter
BBa_K1351025BBa_K1351025 Version 1 (Component)P<sub><i>comAB</i></sub>, a CSP inducible promoter derived from <i>Streptococcus pneumoniae</i>
BBa_K1351024BBa_K1351024 Version 1 (Component)P<sub><i>comC</i></sub>, a CSP inducible promoter derived from <i>Streptococcus pneumoniae</i>
BBa_K730001BBa_K730001 Version 1 (Component)The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide.
BBa_K1687001BBa_K1687001 Version 1 (Component)An enhanced lacI protein generator, inhibits Plac perfectly
cspBBBa_K525224 Version 1 (Component)S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS
BBa_R4030BBa_R4030 Version 1 (Component)PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)
IodoY RSBBa_K1416001 Version 1 (Component)The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon
BBa_K2149019BBa_K2149019 Version 1 (Component)RFP with Lux D and an aldehyde decarbonylase
BBa_S01638BBa_S01638 Version 1 (Component)Intermediate part from assembly 240
BBa_K1179005BBa_K1179005 Version 1 (Component)(Acyl-TyA-rtTA3-His) A membrane/exosome targeting rtTA3 fusion to send through exosomes with His tag
BBa_S03263BBa_S03263 Version 1 (Component)Intermediate part from assembly 317
BBa_S01368BBa_S01368 Version 1 (Component)Intermediate part from assembly 239
BBa_S01370BBa_S01370 Version 1 (Component)Intermediate part from assembly 239
BBa_S01369BBa_S01369 Version 1 (Component)Intermediate part from assembly 239
BBa_K539392BBa_K539392 Version 1 (Component)Vio operon ABDE(each preceded by their own ribosome binding site) with a terminator
BBa_K1351039BBa_K1351039 Version 1 (Component)P<sub><i>xylA</i></sub>, a xylose inducible promoter for <i>Bacillus subtilis</i>
BBa_S01896BBa_S01896 Version 1 (Component)Intermediate part from assembly 240
BBa_S01419BBa_S01419 Version 1 (Component)Intermediate part from assembly 240
BBa_S01535BBa_S01535 Version 1 (Component)Intermediate part from assembly 240
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K658010BBa_K658010 Version 1 (Component)a bacteria population-control device with lux pR-5 driven by lacl+pL
BBa_K658009BBa_K658009 Version 1 (Component)a bacteria population-control device with lux pR-3 driven by lacl+pL
PhyB-908-(BBa_K365011 Version 1 (Component)Phytochrome B (aa 1-908) linked to a trimer of ClpX (aa 61-425)
PB642(L-C)BBa_K365010 Version 1 (Component)Phytochrome B (aa 1-642) linked to a trimer of ClpX (aa 61-425)
BBa_K1051036BBa_K1051036 Version 1 (Component)BBa_K1051105(non stop codon to Mitochondria Outer Membrane)+BBa_K1051008( CAA terminator with stopco
BBa_K1051351BBa_K1051351 Version 1 (Component)K1051301 (clb2 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)