BiosurfactBBa_K653000 Version 1 (Component)Re-designing "The Biosurfactator" The first Central American BioBrick
BBa_K615003BBa_K615003 Version 1 (Component)E. coli strain for TolC one-hybrid selection system
BBa_K850000BBa_K850000 Version 1 (Component)AGM1 gene from candida albicans
BBa_K850001BBa_K850001 Version 1 (Component)NAG1 gene from candida albicans
BBa_K850002BBa_K850002 Version 1 (Component)UAP1 gene from candida albicans
BBa_K748004BBa_K748004 Version 1 (Component)P3+RBS+GFP+T. P3 can be activated by phosphorylated AgrA.
BBa_K748005BBa_K748005 Version 1 (Component)P2+RBS+GFP+T. P2 can be activated by phosphorylated AgrA.
BBa_K748008BBa_K748008 Version 1 (Component)P2 promoter. P2 promoter can be activated by phosphorylated AgrA.
BBa_K748009BBa_K748009 Version 1 (Component)P3 promoter. P3 can be activated by phosphorylated AgrA.
BBa_K748000BBa_K748000 Version 1 (Component)AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.
BBa_K748001BBa_K748001 Version 1 (Component)AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.
BBa_K887011BBa_K887011 Version 1 (Component)DNA program : specific DNA sequence which can be recognized by zinc fingers
BBa_K812013BBa_K812013 Version 1 (Component)GFP-AID OsTirI polysistronic system for auxin detection in tadpole
CBDcexBBa_K863101 Version 1 (Component)Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)
BBa_K737023BBa_K737023 Version 1 (Component)Promoter that can be induced by phosphate.
BBa_K737026BBa_K737026 Version 1 (Component)Promoter that can be induced by phosphate and carbon starvation.
BBa_K737048BBa_K737048 Version 1 (Component)The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42
BBa_K737049BBa_K737049 Version 1 (Component)The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42
BBa_K737050BBa_K737050 Version 1 (Component)The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42
CBDcex(T7)BBa_K863102 Version 1 (Component)Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
BBa_K750008BBa_K750008 Version 1 (Component)Quorum sensing system based on LuxI and LuxR to control the expression of parts behind
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Cel5ABBa_K805011 Version 1 (Component)Cel5A, endoglucanases
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_S04138BBa_S04138 Version 1 (Component)cI QPI with GFP reporter system
BBa_K098988BBa_K098988 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and high promoter
BBa_K098987BBa_K098987 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and low promoter
BBa_K098982BBa_K098982 Version 1 (Component)lac inducible system with low promoter and GFP reporter
rTT+pRBBa_K093003 Version 1 (Component)Convergent Promoter System: Forward Module: rTT+lambda pR
rPlac+TTBBa_K093004 Version 1 (Component)Convergent Promoter System: Reverse Module: rPlac+TT
BBa_K082017BBa_K082017 Version 1 (Component)general recombine system
BBa_K116001BBa_K116001 Version 1 (Component)nhaA promoter, that can be regulated by pH and nhaR protein.
BBa_K116401BBa_K116401 Version 1 (Component)external phosphate sensing promoter
BBa_K153008BBa_K153008 Version 1 (Component)CqsA - the CAI-1 producer
BBa_K127003BBa_K127003 Version 1 (Component)Polystyrene binding peptide inserted CPX generated by qurum sensing and constitutive GFP genarator
agrDBBa_K090601 Version 1 (Component)AgrD: AGR System AIP Precursor
BBa_K116404BBa_K116404 Version 1 (Component)external phosphate sensing reporter
BBa_J37015BBa_J37015 Version 1 (Component)Prey Molecule Generator (AHL) + GFP Reporter
BBa_M11033BBa_M11033 Version 1 (Component)Endo-1,4-beta-glucanase B [Clostridium thermocellum]
BBa_I715069BBa_I715069 Version 1 (Component)Test part to see if RE and pLac +hixc can be expressed with GFP
BBa_M10034BBa_M10034 Version 1 (Component){˂Ag43_short!}, for Ag 43 autotransporter display system, only transporter
BBa_M10035BBa_M10035 Version 1 (Component){˂Ag43_long!} for Ag 43 autotransporter display system, including passenger domain
BBa_K199000BBa_K199000 Version 1 (Component)CCCUC tRNA Suppressor (Produces Serine)
BBa_K199001BBa_K199001 Version 1 (Component)CUAGU tRNA Suppressor (Produces Serine)
BBa_K199002BBa_K199002 Version 1 (Component)CCACU tRNA Suppressor (Produces Serine)
BBa_K294001BBa_K294001 Version 1 (Component)Protein generator that produces E. coli heat shock protein hsp15
BBa_K294031BBa_K294031 Version 1 (Component)protein generator that produces protein hsp15
BBa_K199007BBa_K199007 Version 1 (Component)CCAUC Suppressor tRNA (9-bp Anticodon and Produces Serine)
BBa_K199008BBa_K199008 Version 1 (Component)CCAUC Suppressor tRNA (10-bp Anticodon and Produces Serine)
BBa_K199014BBa_K199014 Version 1 (Component)AGGAC tRNA Suppressor (Produces Serine)