BBa_K1791003
BBa_K1791003 Version 1 (Component)
6X end terminally His tagged MS2 coat protein

BBa_K362007
BBa_K362007 Version 1 (Component)
This composite part has the promoter ,ahpC-promoter controlled by OxyR protein, and GFP - T-T

BBa_J147000
BBa_J147000 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

BBa_J147002
BBa_J147002 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_K1031021
BBa_K1031021 Version 1 (Component)
Hybrid Promoter for Band-pass Filter

BBa_K1456023
BBa_K1456023 Version 1 (Component)
Human Tissue Plasminogen Activator (tPA) enzyme forward primer-2 with restriction site and kozak seq

BBa_K1456021
BBa_K1456021 Version 1 (Component)
Glutathione Peroxidase-1 (GPx-1) enzyme forward primer-2 with restriction site and kozak sequence

BBa_K1179008
BBa_K1179008 Version 1 (Component)
A mutant DNA binding Cas9 with a C-terminal linker and BsaI site for ligation

BBa_K1456022
BBa_K1456022 Version 1 (Component)
Glutathione Peroxidase(GPx1) enzyme reverse primer-2 with restriction site, two stop codon and 6xHis

BBa_K1456024
BBa_K1456024 Version 1 (Component)
Human Tissue Plasminogen Activator reverse primer-2 with restriction site, 2 stop codons and 6XHis

BBa_K079026
BBa_K079026 Version 1 (Component)
GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter

BBa_K079024
BBa_K079024 Version 1 (Component)
LacI repressor under the control of the J23118 costitutive promoter and Lac 1 operator

BBa_K2116038
BBa_K2116038 Version 1 (Component)
AND gate103

BBa_I715069
BBa_I715069 Version 1 (Component)
Test part to see if RE and pLac +hixc can be expressed with GFP

BBa_K1657000
BBa_K1657000 Version 1 (Component)
It is called BAT. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_M11051
BBa_M11051 Version 1 (Component)
Captures light energy and uses it to move protons across the membrane out of the cell.

BBa_K1172304
BBa_K1172304 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of T7 promoter and strong RBS

GFP-AID
BBa_K812110 Version 1 (Component)
GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid

BBa_K541925
BBa_K541925 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and LipA signal peptide

BBa_K379000
BBa_K379000 Version 1 (Component)
AHL induced concentration Band detect

Co-op
BBa_K233002 Version 1 (Component)
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_K233001
BBa_K233001 Version 1 (Component)
Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_K1316009
BBa_K1316009 Version 1 (Component)
yqjF promoter coupled to mKate2 reporter gene and ybiJ promoter coupled to mKate2 reporter gene

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

pLacIQ+eGF
BBa_K193207 Version 1 (Component)
TmTHP with promotor for constituitive expression and with EGFP for fusion of Anti-Freeze Protein

T7+eGFP+Tm
BBa_K193208 Version 1 (Component)
TmTHP with promotor for constituitive expression and with EGFP for fusion of Anti-Freeze Protein

DspB
BBa_K1659200 Version 1 (Component)
Dispersin B, an antibiofilm enzyme that specifically hydrolyses PGA polymers

BBa_K1316020
BBa_K1316020 Version 1 (Component)
2nd part of Constitutive Anderson promoter + csgA:Histag curli gene

BBa_K1316019
BBa_K1316019 Version 1 (Component)
2nd part of Constitutive Anderson promoter + csgA curli gene

BBa_K774102
BBa_K774102 Version 1 (Component)
Multi sensor - for calculation of specific concentrations of nitrates nitrites and nitric oxide usin

BBa_K1412600
BBa_K1412600 Version 1 (Component)
Endow the CL-1(E.coli engineered strain) the ability of chemotaxis and quorum sensing

Hax3-2xNG
BBa_K2075023 Version 1 (Component)
TAL-effector Hax3-2xNG

BBa_K325239
BBa_K325239 Version 1 (Component)
Red Firefly Luciferase and LRE (under pBAD)<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

BBa_K594013
BBa_K594013 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL

BBa_K379002
BBa_K379002 Version 1 (Component)
AHL induced concentration Band detect

BBa_K379001
BBa_K379001 Version 1 (Component)
AHL induced concentration Band detect

BBa_M45760
BBa_M45760 Version 1 (Component)
K208010:M45710, A composite part for AvrA with a Lac promoter and ribosome binding site.

BBa_K539691
BBa_K539691 Version 1 (Component)
promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator

BBa_K1647019
BBa_K1647019 Version 1 (Component)
Cap-AAP2, a improved antimicrobial peptide which can kill the S.mutans selectively and have changeab

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K112223
BBa_K112223 Version 1 (Component)
{a~ihfB!} The ihf beta gene ready to have rbs attached and stop codon, BBb format

BBa_K1657001
BBa_K1657001 Version 1 (Component)
It is called BAT. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K833014
BBa_K833014 Version 1 (Component)
constitutive promoter followed by a lox site with a bidirectional terminator and then a lox site.