BBa_K379001

BBa_K379001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K379001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: syed imtiyaz

Date created: 2010-10-25 11:00:00

Date modified: 2015-05-08 01:12:17

AHL induced concentration Band detect

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K379001_sequence (Version 1)

Description

This component detects the concentration of AHL and activates the reporter protein (GFP/RFP/YFP) when the concentration is in the correct band. If the concentration of AHL is either higher or lower than the band specific to this component, the reporter is not activated.

Sequence and Features

Notes

We had to look for different types of LacI degradation tags to obtain the correct configuration.

Source

lux pL

R0063

.

B0034

luxr

C0062

.

B0010

.

B0012

lux pR

R0062

.

I746352

.

B0010

.

B0012

.

I746361

.

B0034

cI lam

C0051

.

B0010

.

B0012

cI lam

R0051

.

B0034

lacI

C0012

.

B0010

.

B0012

.

I746364

.

B0034

lacI

C0012

.

B0010

.

B0012

LacI

R0010

.

B0034

RFP

E1010

.

B0010

.

B0012

Components

Access	Instance	Definition
public	BBa_R0063	lux pL
public	BBa_B0034	BBa_B0034
public	BBa_C0062	luxr
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_R0062	lux pR
public	BBa_I746352	BBa_I746352
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_I746361	BBa_I746361
public	BBa_B0034	BBa_B0034
public	BBa_C0051	cI lam
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_R0051	cI lam
public	BBa_B0034	BBa_B0034
public	BBa_C0012	lacI
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_I746364	BBa_I746364
public	BBa_B0034	BBa_B0034
public	BBa_C0012	lacI
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_E1010	mRFP1
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0063	1,151	lux pL
BBa_B0034	160,171	BBa_B0034
BBa_C0062	178,933	luxr
BBa_B0010	967,1046	BBa_B0010
BBa_B0012	1055,1095	BBa_B0012
BBa_R0062	1104,1158	lux pR
BBa_I746352	1167,1430	BBa_I746352
BBa_B0010	1439,1518	BBa_B0010
BBa_B0012	1527,1567	BBa_B0012
BBa_I746361	1576,1667	BBa_I746361
BBa_B0034	1676,1687	BBa_B0034
BBa_C0051	1694,2468	cI lam
BBa_B0010	2477,2556	BBa_B0010
BBa_B0012	2565,2605	BBa_B0012
BBa_R0051	2614,2662	cI lam
BBa_B0034	2671,2682	BBa_B0034
BBa_C0012	2689,3816	lacI
BBa_B0010	3850,3929	BBa_B0010
BBa_B0012	3938,3978	BBa_B0012
BBa_I746364	3987,4079	BBa_I746364
BBa_B0034	4088,4099	BBa_B0034
BBa_C0012	4106,5233	lacI
BBa_B0010	5267,5346	BBa_B0010
BBa_B0012	5355,5395	BBa_B0012
BBa_R0010	5404,5603	LacI
BBa_B0034	5612,5623	BBa_B0034
BBa_E1010	5630,6335	mRFP1
BBa_B0010	6344,6423	BBa_B0010
BBa_B0012	6432,6472	BBa_B0012

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K379001/1

Member of these Collections

• iGEM Parts Registry