BBa_K1463753
BBa_K1463753 Version 1 (Component)
MotB and B0032 RBS under J23103 promoter

BBa_K1463703
BBa_K1463703 Version 1 (Component)
MotA and B0032 RBS under J23103 promoter

BBa_I13914
BBa_I13914 Version 1 (Component)
AiiA (+LVA) Tri-part (B0031.C0060.B0015)

BBa_I13912
BBa_I13912 Version 1 (Component)
AiiA (+LVA) Tri-part (B0032.C0060.B0015)

BBa_J119408
BBa_J119408 Version 1 (Component)
Pupp promoter mutant - Substitution of C and G to A at 28 and 30

BBa_K202004
BBa_K202004 Version 1 (Component)
Hybrid promoter having multiple operator sites. Promoter has tetO2 with mutation at position 3

BBa_K1088059
BBa_K1088059 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1088052
BBa_K1088052 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1178000
BBa_K1178000 Version 1 (Component)
tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon

BBa_J70621
BBa_J70621 Version 1 (Component)
RFC12 7x His Tag Tail Domain

BBa_J70459
BBa_J70459 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - B0031 simulator (reverse oligo)

IodoY RS
BBa_K1416001 Version 1 (Component)
The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon

BBa_J06551
BBa_J06551 Version 1 (Component)
Construction intermediate: lambda cI with RBS and hybrid LacI promoter (R0011.B0034.C0051)

BBa_I0414
BBa_I0414 Version 1 (Component)
RBS Test R0040.B0032.E0030.B0015 RBS Test

BBa_K1845001
BBa_K1845001 Version 1 (Component)
Miraculin (Yeast codon optimised + His-tag)

BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive

BBa_I721000
BBa_I721000 Version 1 (Component)
Strong RBS, ECFP (no LVA tag)

BBa_J107021
BBa_J107021 Version 1 (Component)
aTc sensor (J23106 promoter) with GFP

BBa_I724005
BBa_I724005 Version 1 (Component)
Elowitz repressilator with added degradation tag

BBa_K331011
BBa_K331011 Version 1 (Component)
Catechol 2,3-dioxygenase with a C-terminus arginine tag

BBa_K1685002
BBa_K1685002 Version 1 (Component)
aeBlue with LVA tag and double terminator

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K1974011
BBa_K1974011 Version 1 (Component)
T7 Promoter+RBS+Hv1a+linker+6X His-Tag

BBa_K1974013
BBa_K1974013 Version 1 (Component)
T7 Promoter+RBS+OAIP+linker+6X His-Tag

BBa_K346025
BBa_K346025 Version 1 (Component)
PmerT promoter mutant 88+RBS(B0030)+GFP(E0040)Terminator(B0010)+Terminator(B0012)

BBa_K542006
BBa_K542006 Version 1 (Component)
pBAD Inverse-Regulated Arg-tagged ECFP and EYFP (FRET Reporter)

BBa_K1974022
BBa_K1974022 Version 1 (Component)
T7Promoter+RBS+Sf1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974021
BBa_K1974021 Version 1 (Component)
T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974023
BBa_K1974023 Version 1 (Component)
T7Promoter+RBS+OAIP+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1036003
BBa_K1036003 Version 1 (Component)
lux pL controlled luxR with lux pR controlled gfp (LVA-tag)

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.