BBa_K563056
BBa_K563056 Version 1 (Component)
Tor with mutation L1415P in yeast vector pDHBtor

BBa_K112105
BBa_K112105 Version 1 (Component)
BamHI endonuclease without start or stop codons

BBa_K1334028
BBa_K1334028 Version 1 (Component)
IPTG activated promoter similar to R0011

BBa_K415019
BBa_K415019 Version 1 (Component)
pLuxR/cI-OR : RBS : LuxI

BBa_I9111
BBa_I9111 Version 1 (Component)
test (lambda-cI nor) I9110.I9109.I9109

BBa_I9108
BBa_I9108 Version 1 (Component)
test (tp901-cI nor) I9107.I9106.I9106

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J64974
BBa_J64974 Version 1 (Component)
small molecule controlled, riboregulator based XOR gate

BBa_K2044006
BBa_K2044006 Version 1 (Component)
Based on our project,<3,6> is the direct pathway from Site No.3 to Site No.6 in the map we design.

BBa_K2044004
BBa_K2044004 Version 1 (Component)
Based on our project, <2,4> is the direct pathway from Site No.2 to Site No.4 in the map we design.

BBa_K2044009
BBa_K2044009 Version 1 (Component)
Based on our project, <4,8> is the direct pathway from Site No.4 to Site No.8 in the map we design.

BBa_K2044014
BBa_K2044014 Version 1 (Component)
Based on our project, <1,4> is the direct pathway from Site No.1 to Site No.4 in the map we design.

BBa_K2044007
BBa_K2044007 Version 1 (Component)
Based on our project, <4,5> is the direct pathway from Site No.4 to Site No.5 in the map we design.

BBa_K2044013
BBa_K2044013 Version 1 (Component)
Based on our project,<7,8> is the direct pathway from Site No.7 to Site No.8 in the map we design.

BBa_K2044008
BBa_K2044008 Version 1 (Component)
Based on our project, <4,6> is the direct pathway from Site No.4 to Site No.6 in the map we design.

BBa_K2044003
BBa_K2044003 Version 1 (Component)
Based on our project, <2,3> is the direct pathway from Site No.2 to Site No.3 in the map we design.

BBa_K2044002
BBa_K2044002 Version 1 (Component)
Based on our project, <2,1> is the direct pathway from Site No.2 to Site No.1 in the map we design.

BBa_K2044005
BBa_K2044005 Version 1 (Component)
Based on our project, <2,6> is the direct pathway from Site No.2 to Site No.6 in the map we design.

BBa_K2044012
BBa_K2044012 Version 1 (Component)
Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.

BBa_K2044011
BBa_K2044011 Version 1 (Component)
Based on our project,<6,4> is the direct pathway from Site No.6 to Site No.4 in the map we design.

BBa_M12101
BBa_M12101 Version 1 (Component)
PTet : B0034 : GFP in BioScaffold Form, a [0,5;7,2) BioScaffold

BBa_K239003
BBa_K239003 Version 1 (Component)
Modified HypB promoter, activated by Fnr during oxygen deficiency

BBa_M1183
BBa_M1183 Version 1 (Component)
Practice for Class

BBa_K415022
BBa_K415022 Version 1 (Component)
PluxR/cI-OR : RBS : mCherry : Term : PtetR : RBS : LuxR : Term : Plux/cI-OR : RBS : LuxI

BBa_M1265
BBa_M1265 Version 1 (Component)
Gluteomorphin or gliadorphin

BBa_M1264
BBa_M1264 Version 1 (Component)
gluteomorphin or gliadorphin

BBa_K2116029
BBa_K2116029 Version 1 (Component)
weak RBS for bxb1

CDA
BBa_K1266000 Version 1 (Component)
Chitin Deacetylase or CDA2

BBa_J70406
BBa_J70406 Version 1 (Component)
R0010.J70400 (Ribosome Replacer or Tag Adder)

BBa_J119316
BBa_J119316 Version 1 (Component)
Scaffold 2.0 for J-GGA (With the promoter between Junction A & B and the RBS in the junction B & C)

BBa_M36731
BBa_M36731 Version 1 (Component)
EfeU elemental iron (II) transporter + 5' UTR (medium) + terminator + Fur promoter

BBa_J70655
BBa_J70655 Version 1 (Component)
RFP optimized for expression in E. coli and M. florum

BBa_K427008
BBa_K427008 Version 1 (Component)
Ter PBadweak Mu Mor Ter PM GFP cassette

BBa_K1155006
BBa_K1155006 Version 1 (Component)
Promoter of narK activated by FNR in anaerobic condition

P(narG)
BBa_K1155005 Version 1 (Component)
Promoter of narG activated by FNR in anaerobic conditions

P(nirB)
BBa_K1155004 Version 1 (Component)
Promoter of nirB, activated by FNR in anaerobic conditions

<partinfo>
BBa_K1697001 Version 1 (Component)
moved to <partinfo>BBa_K1697002 </partinfo>

BBa_M1638
BBa_M1638 Version 1 (Component)
LacSwap + GFP for testing

BBa_K1431412
BBa_K1431412 Version 1 (Component)
target sequence1 for HBV

BBa_K549005
BBa_K549005 Version 1 (Component)
LacI promotor fused with the iron dependent regulator fur

BBa_K239004
BBa_K239004 Version 1 (Component)
Part of narGP promoter, activated by Fnr during oxygen deficiency

BBa_K1351022
BBa_K1351022 Version 1 (Component)
FsrA: Fur-regulated sRNA which binds the binding site sdhC

BBa_K1132004
BBa_K1132004 Version 1 (Component)
XOR gate with recombinases switching gene regulatory sequences and ORF

BBa_M31540
BBa_M31540 Version 1 (Component)
Four Proteins involved in the production of the M13K07 phage coat.

BBa_J34200
BBa_J34200 Version 1 (Component)
XOR gate, 2x PoPS input, 1x PoPS output

BBa_K115056
BBa_K115056 Version 1 (Component)
IPP -> OPP or DMAPP -> OPP

BBa_I8023
BBa_I8023 Version 1 (Component)
Booperator test: Inverter NOR biasing in polycistronic design

BBa_I8022
BBa_I8022 Version 1 (Component)
Booperator test: Inverter NOR biasing in monocistronic design

BBa_J95039
BBa_J95039 Version 1 (Component)
plasmid vector for Rhodobacter sphaeroides

BBa_J95025
BBa_J95025 Version 1 (Component)
promoter designed for Rhodobacter sphaeroides