BBa_J01120BBa_J01120 Version 1 (Component)Sacrafical OnRFP Kan vector
BBa_K109551BBa_K109551 Version 1 (Component)TrbA transcription factor for the Tra2 operon in incP alpha conjugation
BBa_K112995BBa_K112995 Version 1 (Component)BBb1 assembly vector - C/A
BBa_K1531005BBa_K1531005 Version 1 (Component)Bovine kappa casein (B variant) with full alpha-factor yeast secretion signal
BBa_K1531004BBa_K1531004 Version 1 (Component)Bovine beta casein (B variant) with full alpha-factor yeast secretion signal
BBa_K1172913BBa_K1172913 Version 1 (Component)Part 2 of the Biosafety-System TetOR alive (TetO Barnase)
BBa_K1351040BBa_K1351040 Version 1 (Component)pBS0K<i>Pspac</i>, an IPTG-inducible replicative expression vector for
BBa_J72117BBa_J72117 Version 1 (Component)BBb High copy entry vector, pBca1256
BBa_K1172912BBa_K1172912 Version 1 (Component)Part 1 of the Biosystem TetOR alive (pRha TetR alr)
BBa_K1172914BBa_K1172914 Version 1 (Component)Part 2 of the Biosafety-System TetOR alive (TetO GFP)
BBa_M36745BBa_M36745 Version 1 (Component)The sensor codes for AraC, a transcription factor, which represses the operon.Arabinose induces tran
BBa_K106693BBa_K106693 Version 1 (Component)AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)
BBa_M36475BBa_M36475 Version 1 (Component)Codes for AraC, a transcription factor which represses operon. Arabinose dislodges TF protein.
BBa_K086002BBa_K086002 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP
BBa_K086008BBa_K086008 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP
BBa_K086006BBa_K086006 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP
BBa_K086005BBa_K086005 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP
BBa_K086009BBa_K086009 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP
BBa_K1114400BBa_K1114400 Version 1 (Component)This is a MoClo level 0 destination vector.
BBa_K086001BBa_K086001 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter
BBa_K086012BBa_K086012 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter
BBa_K086007BBa_K086007 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP reporter
BBa_K086013BBa_K086013 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter
BBa_K086004BBa_K086004 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP promoter
BBa_K086014BBa_K086014 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter
BBa_K086016BBa_K086016 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter
BBa_K086011BBa_K086011 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter
BBa_K086003BBa_K086003 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter
BBa_K086010BBa_K086010 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter
BBa_K086015BBa_K086015 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter
BBa_K1039021BBa_K1039021 Version 1 (Component)Bxb1 integrase and Recombination Directionality Factor (RDF) Under ???Lock and Key??? Control with L
BBa_J72078BBa_J72078 Version 1 (Component){double terminator}, b0015 in BglBrick format
BBa_M11410BBa_M11410 Version 1 (Component)Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse
BBa_K1033204BBa_K1033204 Version 1 (Component)pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance
BBa_K563053BBa_K563053 Version 1 (Component)vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
BBa_K802003BBa_K802003 Version 1 (Component)Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>
BBa_K1363200BBa_K1363200 Version 1 (Component)Anti-LPS factor(LALF) regulated by lacI
BBa_K2092004BBa_K2092004 Version 1 (Component)alcR (incl RBS), ethanol-activated transcription factor from A. nidulans
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.