Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
mRFP1BBa_E1010 Version 1 (Component)**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
BBa_I712017BBa_I712017 Version 1 (Component)Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.
BBa_K1321340BBa_K1321340 Version 1 (Component)Double CBD (dCBD) with N-terminal linker
GFP1BBa_I715019 Version 1 (Component)Amino Half of GFP (aka GFP1)
BBa_J56012BBa_J56012 Version 1 (Component)Invertible sequence of dna includes Ptrc promoter
GFP2BBa_I715020 Version 1 (Component)Carboxyl Half of GFP (aka GFP2)
BLpBBa_K238013 Version 1 (Component)Exact promoter of the blue light receptor
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BBa_K249008BBa_K249008 Version 1 (Component)YFP with N-terminal Arganine Tag
LacIBBa_K143033 Version 1 (Component)LacI (Lva<sup>-</sup>, N-terminal deletion) regulatory protein
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
BBa_M1652BBa_M1652 Version 1 (Component)CXC chemokine BRAK mRNA
pSC101BBa_I50041 Version 1 (Component)pSC101 origin of replication, reverse
rhIIBBa_C0070 Version 1 (Component)autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
BclA-HisBBa_K1933000 Version 1 (Component)N-terminal domain of BclA protein fused to 6xHis tag
BBa_K1423000BBa_K1423000 Version 1 (Component)BCLA N-terminal Domain
BBa_K1362173BBa_K1362173 Version 1 (Component)Split sfGFP reconstitution N-terminal splicing half
BBa_K1728021BBa_K1728021 Version 1 (Component)Chemokine receptor 1 (CXCR1)
BBa_K777000BBa_K777000 Version 1 (Component)Tar receptor
G-His6(N)BBa_K1362430 Version 1 (Component)C-terminal His6 (N-Intein)
RFC105 NNBBa_K1362415 Version 1 (Component)N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN
BBa_K1362174BBa_K1362174 Version 1 (Component)Split sfGFP reconstitution N-terminal non-splicing control half
OmpABBa_K103006 Version 1 (Component)OmpA outer membrane protein A fused to linker; displays proteins on cell surface
lacZ aBBa_E0033 Version 1 (Component)LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
His-ScaffoBBa_K1602027 Version 1 (Component)Protein scaffold with N-terminal HisTag
BBa_J44001BBa_J44001 Version 1 (Component)Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
BBa_K331001BBa_K331001 Version 1 (Component)ECFP with N-terminal Arginine Tag
BBa_K1159101BBa_K1159101 Version 1 (Component)N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]
His6BBa_K1362431 Version 1 (Component)N-terminal His6 (C-Intein)