BBa_K861500
BBa_K861500 Version 1 (Component)
FadL, Long-chain fatty acids transporter from <i>E.coli str. K12</i>

BBa_K797001
BBa_K797001 Version 1 (Component)
phage shock protein A: one of the components of out Tat cassette

BBa_K1911003
BBa_K1911003 Version 1 (Component)
ClpX from E. Coli

BBa_K1911002
BBa_K1911002 Version 1 (Component)
ClpP from E. coli

BBa_M36627
BBa_M36627 Version 1 (Component)
GFP (modified from BBa_K648013)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J70102
BBa_J70102 Version 1 (Component)
Left 6 his test part for library construction part.

BBa_J70104
BBa_J70104 Version 1 (Component)
Right 6 his test part for library construction part.

BBa_K1332003
BBa_K1332003 Version 1 (Component)
The 5?? side of the intron(+exon fragment) from td gene of T4 phage without stop codon

BBa_J22102
BBa_J22102 Version 1 (Component)
rec A (SOS) Promoter

BBa_K258100
BBa_K258100 Version 1 (Component)
PrtD domain of ABC transporter of Erwinia chrysanthemi

BBa_M36028
BBa_M36028 Version 1 (Component)
phiX174 gene D - mutated rbs and start codon of E, and rbs of J

BBa_I11052
BBa_I11052 Version 1 (Component)
P21 attB rev comp

BBa_K199176
BBa_K199176 Version 1 (Component)
pT7+RBS+GFP

BBa_K112140
BBa_K112140 Version 1 (Component)
attR1 rev-CamR-Lox-AmpR

AatA
BBa_K901009 Version 1 (Component)
AatA from <i>Acetobacter acetii</i>

BBa_I13406
BBa_I13406 Version 1 (Component)
Pbad/AraC with extra REN sites

BBa_K1232000
BBa_K1232000 Version 1 (Component)
Green light inducible sfGFP = [PCpcG2-RBS-sfGFP-CcaS](rev)-CcaR

HfsG
BBa_K196002 Version 1 (Component)
HfsG protein from <i>Caulobacter crescentus</i>

HfsH
BBa_K196003 Version 1 (Component)
HfsH protein from <i>Caulobacter crescentus</i>

BBa_K414003
BBa_K414003 Version 1 (Component)
Strong Plant RBS (Kozak sequence) + mCherry from J06504

BBa_K414004
BBa_K414004 Version 1 (Component)
Strong Plant RBS (Kozak sequence) + EYFP from E0030

BBa_J119374
BBa_J119374 Version 1 (Component)
tClone TetA: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly

BBa_J70034
BBa_J70034 Version 1 (Component)
A BioScaffold Part (Uses AarI) for Library Vector Preparation (see Part Design page)

Down
BBa_K1642006 Version 1 (Component)
Downstream sequence from slr0168

Up
BBa_K1642005 Version 1 (Component)
Upstream sequence from slr0168

BBa_I20297
BBa_I20297 Version 1 (Component)
RBS from T7 gene 4 + Z0301 (venus)

BBa_J61028
BBa_J61028 Version 1 (Component)
[P_T7][Tn5-rev]

BBa_K133013
BBa_K133013 Version 1 (Component)
CF215-RGD-Histop

BBa_K133017
BBa_K133017 Version 1 (Component)
mCherry-RGD-Histop

BBa_K315042
BBa_K315042 Version 1 (Component)
pLac-RBS-RFP-RBS-TetA (Deoptimized from bp 1-290)

BBa_K315043
BBa_K315043 Version 1 (Component)
pLacI-RBS-RFP-RBS-TetA (Optimized from bp 1-290)

BBa_K315031
BBa_K315031 Version 1 (Component)
pLac-RBS-RFP-RBS-TetA(Optimized from bp 1-290)

BBa_K315044
BBa_K315044 Version 1 (Component)
pLacI-RBS-RFP-RBS-TetA (Deoptimized from bp 1-290)

BBa_K118028
BBa_K118028 Version 1 (Component)
beta-glucosidase gene bglX (chu_2268) from Cytophaga hutchinsonii

raiR
BBa_K594008 Version 1 (Component)
a luxR-type regulator from Rhizobium etli CFN42

BBa_K511815
BBa_K511815 Version 1 (Component)
Inducible Red Fluorescent Protein Generator (TRE-Tight-mKate) MammoBlock Device

BBa_K511807
BBa_K511807 Version 1 (Component)
Repressible Red Fluorescent Protein Generator (Hef1a-LacO-mKate) MammoBlock Device

BBa_K511802
BBa_K511802 Version 1 (Component)
Red Fluorescent Delta Ligand Generator (Hef1a-Delta-mCherry) MammoBlock Device

BBa_K511823
BBa_K511823 Version 1 (Component)
Inducible Red Fluorescent Protein Generator (UAS-Gal4-mKate) MammoBlock Device

BBa_K1026004
BBa_K1026004 Version 1 (Component)
A gRNA targeting mRFP, negatively regulated by Red Light Sensor

SPtom40
BBa_K809204 Version 1 (Component)
signal peptide of Tom40

BBa_K150005
BBa_K150005 Version 1 (Component)
ribosome binding site of pTrc99a

tetR
BBa_Q01400 Version 1 (Component)
QPI (TetR, Medium RBS)

BBa_K1087006
BBa_K1087006 Version 1 (Component)
delta activator from phiR73 phage without RBS but with B0015

BBa_K1983013
BBa_K1983013 Version 1 (Component)
Phenylalanine-specific permease (PheP) from Escherichia coli

BBa_J176071
BBa_J176071 Version 1 (Component)
EED expression casette 1