BBa_K1395000
BBa_K1395000 Version 1 (Component)
nrfA gene (Nitrite reductase enzyme) under constitutive promoter

BBa_K1199046
BBa_K1199046 Version 1 (Component)
DhaA31-P2A-HheCW249P 1,2,3-tricholoropropane(TCP)->Glycerol

BBa_K324002
BBa_K324002 Version 1 (Component)
Tat protein transduction domain with glycine linker (RFC25)

BBa_K541546
BBa_K541546 Version 1 (Component)
IPTG inducible PlacI, RBS,TAT signal peptide and reflectin

BBa_K541536
BBa_K541536 Version 1 (Component)
Reflectin 1A for E.coli with TAT signal sequence (Constitutive)

BBa_K1202115
BBa_K1202115 Version 1 (Component)
Tat Apoptin For Bacterial Secretion With TorA Signal Peptide

BBa_K2122200
BBa_K2122200 Version 1 (Component)
His-tagged Shiga-Like Subunit B toxin

BBa_K1621004
BBa_K1621004 Version 1 (Component)
gag/tat/pol/env - polyepitopic antigen derived from HIV-1

LacIm
BBa_K092800 Version 1 (Component)
Coding sequence for LacI modified with a different rate of translation than LacI, RBS

BBa_K1444011
BBa_K1444011 Version 1 (Component)
Composite promoter and consensus B. subtilis RBS - pTetR

BBa_J92006
BBa_J92006 Version 1 (Component)
M. Ruminantium RNA polymerase Subunit A"

cspB
BBa_K525224 Version 1 (Component)
S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS

BBa_K594011
BBa_K594011 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.

BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

BBa_I761013
BBa_I761013 Version 1 (Component)
Insulin A generator with TAT signal peptide with glucose-inducible promoter

BBa_K1444014
BBa_K1444014 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - pTetR x2

BBa_K1444010
BBa_K1444010 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - C1-434

BBa_I20292
BBa_I20292 Version 1 (Component)
There is no limit to what a man can do or where he can go if...

BBa_K1932006
BBa_K1932006 Version 1 (Component)
This device is constructed to express TAT-apoptin fused with sec2.

BBa_K1938013
BBa_K1938013 Version 1 (Component)
Chlorite dismutase with Constitutive Strong promoter, Strong RBS and TAT signal peptide

BBa_K1067000
BBa_K1067000 Version 1 (Component)
Periplasmic directed GFP SF with signal peptide TAT and RFP as background color

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

CspB | RFP
BBa_K525234 Version 1 (Component)
Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS

BBa_K525264
BBa_K525264 Version 1 (Component)
Fusion Protein of Luciferase, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7

BBa_K525261
BBa_K525261 Version 1 (Component)
Fusion Protein of Luciferase and cspB from Corynebacterium halotolerans with TAT-Sequence and lipid

cspB
BBa_K525221 Version 1 (Component)
S-layer cspB from Corynebacterium halotolerans with TAT-Sequence and lipid anchor, PT7 and RBS

cspB
BBa_K525121 Version 1 (Component)
S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS

BBa_K2170132
BBa_K2170132 Version 1 (Component)
C-terminal intermediate of eukaryotic hypoxie sensor (VEGF-p2A-PDGF_polyA) in RFC[10]

BBa_J58008
BBa_J58008 Version 1 (Component)
Periplasmic binding protein that docks a vanillin molecule

BBa_K1932007
BBa_K1932007 Version 1 (Component)
This device is constructed for the expression of TAT-apoptin fused with tmp1.

BBa_K323164
BBa_K323164 Version 1 (Component)
VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5

BBa_K323163
BBa_K323163 Version 1 (Component)
VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter in pSB4C5

BBa_K1412088
BBa_K1412088 Version 1 (Component)
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.