BBa_I741107BBa_I741107 Version 1 (Component)xylA and xylB metabolizing proteins plus region inbetween (with silent mutations)
BBa_K2170202BBa_K2170202 Version 1 (Component)StrepTag for interaction with biotin binding poteins in RFC[25]
BBa_M36938BBa_M36938 Version 1 (Component)Enoyl-[acyl-carrier-protien] reductase actuator with GFP reporter
BBa_K1897033BBa_K1897033 Version 1 (Component)superfolded Green Fluorescent Protein
BBa_K116503BBa_K116503 Version 1 (Component)GFP under control of pOmpC promoter.R0082+E0240
BBa_K1904005BBa_K1904005 Version 1 (Component)Fused protein (Holin/Endolysin) with RBS and terminator, C-terminal HisTag, (RFC 25 N-Part).
BBa_K1179006BBa_K1179006 Version 1 (Component)A backbone for ligation of the Acyl-TyA domain to a protein of your choice
BBa_K758000BBa_K758000 Version 1 (Component)UAS , this is the enhancer . And this enhancer is used to identify the protein localization .
BBa_J70652BBa_J70652 Version 1 (Component)This tests adding an internal strep tag linker to a protein using J70590 as a te
BBa_K1465208BBa_K1465208 Version 1 (Component)Carbonic anhydrase (csoS3) and shell-proteins (csoS14) of the carboxysome of H. neapolitanus
BBa_K1051801BBa_K1051801 Version 1 (Component)Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib
BBa_K900003BBa_K900003 Version 1 (Component)Vacuolar Membrane Targeting Protein
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K1179000BBa_K1179000 Version 1 (Component)(Acyl-TyA) A membrane targeting domain for exosomal transportation of proteins
AmphiphiliBBa_K1655002 Version 1 (Component)Amphiphilic proteins can be used to form intracellular micelles or vesicles.
BBa_K563053BBa_K563053 Version 1 (Component)vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
BBa_K362007BBa_K362007 Version 1 (Component)This composite part has the promoter ,ahpC-promoter controlled by OxyR protein, and GFP - T-T
BBa_K1778001BBa_K1778001 Version 1 (Component)TRE is closely related to promoter regulation,can correctly specific binding to regulatory protein r
BBa_K1088002BBa_K1088002 Version 1 (Component)Reporter system to quantify protein expression of B. sub 1-deoxyxylulose-5-phosphate synthase (Dxs)
BBa_M1532BBa_M1532 Version 1 (Component)Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP
BBa_M1533BBa_M1533 Version 1 (Component)Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP
BBa_K079026BBa_K079026 Version 1 (Component)GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter
BBa_K1162307BBa_K1162307 Version 1 (Component)WAM-1 antimicrobial peptide fused with 8x spider silk protein, with 10x His-Tag
BBa_K1413045BBa_K1413045 Version 1 (Component)A fusion of Universal Transposon Plasmid and pSB1C3
BBa_M45016BBa_M45016 Version 1 (Component)phasin protein
BBa_J70653BBa_J70653 Version 1 (Component)This tests adding an internal strep tag linker to a protein using J70590 as a te
BBa_K541925BBa_K541925 Version 1 (Component)Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and LipA signal peptide
BBa_K1431301BBa_K1431301 Version 1 (Component)TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein
BBa_K2097000BBa_K2097000 Version 1 (Component)CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.
BBa_K2082231BBa_K2082231 Version 1 (Component)RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434
BBa_K431002BBa_K431002 Version 1 (Component)Rattus norvegicus monoamine oxidase A (Maoa), nuclear gene encoding mitochondrial protei
PcotVBBa_K823033 Version 1 (Component)P<sub><i>cotV</i></sub>: <i>B. subtilis</i> promoter regulates spore crust protein cotV
BBa_K1217012BBa_K1217012 Version 1 (Component)Eut Microcompartment proteins (EutSMNLK) from BBa_K311004 with His tag fused to N-terminal of EutS
BBa_K258016BBa_K258016 Version 1 (Component)Vhb protein
BBa_K2018037BBa_K2018037 Version 1 (Component)phaCAB genes with additional promoter, that encodes proteins that can create PHB.
BBa_K2018039BBa_K2018039 Version 1 (Component)phaCAB genes with additional promoter, that encodes proteins that can create PHB.
BBa_K2018038BBa_K2018038 Version 1 (Component)phaCAB genes with additional promoter, that encodes proteins that can create PHB.
BBa_K861160BBa_K861160 Version 1 (Component)CRP, cAMP receptor protein
BBa_K079049BBa_K079049 Version 1 (Component)GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator
BBa_K079020BBa_K079020 Version 1 (Component)GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein
BBa_M1530BBa_M1530 Version 1 (Component)Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP
BBa_M1515BBa_M1515 Version 1 (Component)Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP