BBa_K1895005
BBa_K1895005 Version 1 (Component)
Controlled OrpF Expression Device for Microbial Fuel Cell Use

BBa_K631037
BBa_K631037 Version 1 (Component)
GPC-1:eCFP:UNC-54 (for use in C. elegans)

BBa_K1796115
BBa_K1796115 Version 1 (Component)
acetyl-Coenzyme A synthase Alpha subunit C-terminal domain

NO Sensor
BBa_M36210 Version 1 (Component)
Nitric oxide sensing construct for use in Bacillus subtilis

BBa_K812120
BBa_K812120 Version 1 (Component)
IaaH on PSC2+ Ready for use in xenopus tropicalis egg

IBMc334
IBMc334 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-FKBP-T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter

IBMc335
IBMc335 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter

Propane1
BBa_K1655000 Version 1 (Component)
Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli.

BBa_K309030
BBa_K309030 Version 1 (Component)
eYFP::unc-54 3'UTR (for use in C. elegans)

BBa_I13563
BBa_I13563 Version 1 (Component)
BioBrick site flanked by RNase sites and hairpins for use in Screening Plasmids

BBa_K309021
BBa_K309021 Version 1 (Component)
pStr-220::eCFP::3'UTR (for use in C. elegans)

BBa_K1961000
BBa_K1961000 Version 1 (Component)
CYP2A13 is a member of the Cytochrome P450 (CYP) enzymes family, which are critical for the metaboli

MfeI
BBa_B0047 Version 1 (Component)
MfeI restriction enzyme site (EcoRI compatible overhang)

BBa_K204051
BBa_K204051 Version 1 (Component)
3O₆HSL receiver

AvrII
BBa_B0048 Version 1 (Component)
AvrII restriction enzyme site (XbaI, SpeI compatible overhang)

BBa_K1088015
BBa_K1088015 Version 1 (Component)
IPTG controlled B. sub. dxs coupled with IspG from E. coli

BBa_J5530
BBa_J5530 Version 1 (Component)
Cell-See-Us Bacterial Thermometer

BBa_K1179017
BBa_K1179017 Version 1 (Component)
A mutant DNA binding Cas9 with an N-terminal linker and BsaI site for ligation

BBa_K1179008
BBa_K1179008 Version 1 (Component)
A mutant DNA binding Cas9 with a C-terminal linker and BsaI site for ligation

IBMc360
IBMc360 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-G-M86(1-100)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(101-154)-S-mCherry(193-236)-H6-T

BBa_K309032
BBa_K309032 Version 1 (Component)
pMec-7::eCFP::unc-54 3' UTR (for use in C. elegans chassis)

BBa_M36914
BBa_M36914 Version 1 (Component)
Codes for the benzylalcohol acetyltransferase enzyme

BBa_K309022
BBa_K309022 Version 1 (Component)
pOdr-10::eCFP::unc-54 3'UTR (for use in C. elegans chassis)

BBa_K1717173
BBa_K1717173 Version 1 (Component)
Keratinase US under control of LacI promoter.

IBMc378
IBMc378 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-17)-PI-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(18-154)-S-mCherry*(193-236)-H6-T

IBMc379
IBMc379 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-22)-LG-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(23-154)-S-mCherry*(193-236)-H6-T

IBMc380
IBMc380 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-39)-LD-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(40-154)-S-mCherry*(193-236)-H6-T

BBa_K1088002
BBa_K1088002 Version 1 (Component)
Reporter system to quantify protein expression of B. sub 1-deoxyxylulose-5-phosphate synthase (Dxs)

BBa_K1395000
BBa_K1395000 Version 1 (Component)
nrfA gene (Nitrite reductase enzyme) under constitutive promoter

BBa_K145105
BBa_K145105 Version 1 (Component)
cI under T7 and P_R dual promotor

BBa_S03641
BBa_S03641 Version 1 (Component)
HixC : TetB-RBS_rev-HixC

BBa_K204034
BBa_K204034 Version 1 (Component)
3O₆HSL receiver +GFP

BBa_S03638
BBa_S03638 Version 1 (Component)
HixC-pBad_rev-HixC : RBS-TetF

BBa_S03674
BBa_S03674 Version 1 (Component)
(-1,2) HixC-pBad_rev-HixC : RBS-TetF-HixC

BBa_K145112
BBa_K145112 Version 1 (Component)
cI under T7 and PR_R dual promotor

BBa_K323164
BBa_K323164 Version 1 (Component)
VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5

BBa_K323163
BBa_K323163 Version 1 (Component)
VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter in pSB4C5

BBa_K2020051
BBa_K2020051 Version 1 (Component)
wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.