BBa_J72182
BBa_J72182 Version 1 (Component)
repA expression cassette

BBa_J72183
BBa_J72183 Version 1 (Component)
GFP Expression cassette

BBa_J72184
BBa_J72184 Version 1 (Component)
RepA Expression Cassette

BBa_J72185
BBa_J72185 Version 1 (Component)
GFP expression cassette

BBa_J72186
BBa_J72186 Version 1 (Component)
TrimR, pir expression cassette for genomic integration

BBa_J72188
BBa_J72188 Version 1 (Component)
weak violacein expression cassette

BBa_J72189
BBa_J72189 Version 1 (Component)
strong violacein expression cassette

BBa_J72190
BBa_J72190 Version 1 (Component)
neuS expression cassette

BBa_J72191
BBa_J72191 Version 1 (Component)
wbbL expression cassette

BBa_J72192
BBa_J72192 Version 1 (Component)
manB and neuS expression cassette

BBa_J72193
BBa_J72193 Version 1 (Component)
manB expression cassette

BBa_J72194
BBa_J72194 Version 1 (Component)
neuS expression cassette

BBa_J72195
BBa_J72195 Version 1 (Component)
Barnase/Barstar expression cassette

BBa_J72199
BBa_J72199 Version 1 (Component)
wbbL neuS expression cassette

BBa_J72201
BBa_J72201 Version 1 (Component)
BamHI endonuclease expression cassette

BBa_K733002
BBa_K733002 Version 1 (Component)
<i>xylR</i>+<i>PxylA</i>: A xylose inducible promoter with its transcriptional regulator.

BBa_K812014
BBa_K812014 Version 1 (Component)
Auxin production device for expression in tadpole

BBa_K737004
BBa_K737004 Version 1 (Component)
Inducible GVPA Generator

BBa_K737005
BBa_K737005 Version 1 (Component)
Inducible GVPC Generator

BBa_K750000
BBa_K750000 Version 1 (Component)
pBADGLT:unstable gfp expression device activated by arabinose

BBa_K750001
BBa_K750001 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)

BBa_K750002
BBa_K750002 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)

BBa_K750003
BBa_K750003 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)

BBa_K750004
BBa_K750004 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)

BBa_K737058
BBa_K737058 Version 1 (Component)
Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose

BBa_K737051
BBa_K737051 Version 1 (Component)
IPTG inducible GFP Generator

BBa_K737052
BBa_K737052 Version 1 (Component)
aTc inducible GFP Generaror

BBa_K750005
BBa_K750005 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)

BBa_K750006
BBa_K750006 Version 1 (Component)
LuxR expression device activated by arabinose

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K759001
BBa_K759001 Version 1 (Component)
Aggregation Module inducible by arabinose in E.coli.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K098992
BBa_K098992 Version 1 (Component)
mtrB, high expression

BBa_K098990
BBa_K098990 Version 1 (Component)
mtrB, low expression

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K098010
BBa_K098010 Version 1 (Component)
HO-pcyA, low expression

BBa_K131019
BBa_K131019 Version 1 (Component)
LuxO inducible RFP

BBa_K131026
BBa_K131026 Version 1 (Component)
AHL inducible GFP

BBa_K090501
BBa_K090501 Version 1 (Component)
Gram-Positive IPTG-Inducible Promoter

BBa_K090502
BBa_K090502 Version 1 (Component)
Gram-Positive Xylose-Inducible Promoter

BBa_K143077
BBa_K143077 Version 1 (Component)
P43-gsiB GFP expression construct

BBa_K143078
BBa_K143078 Version 1 (Component)
P43-spoVG GFP expression construct

BBa_K143079
BBa_K143079 Version 1 (Component)
Pveg-spoVG GFP expression construct

BBa_K143080
BBa_K143080 Version 1 (Component)
P43-gsiB RFP expression construct

BBa_K143081
BBa_K143081 Version 1 (Component)
P43-spoVG RFP expression construct