BBa_K833002BBa_K833002 Version 1 (Component)mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K833006BBa_K833006 Version 1 (Component)Mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K833008BBa_K833008 Version 1 (Component)Mutated prfA-UTR thermosensor from Listeria monocytogenes
BBa_K2066500BBa_K2066500 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
BBa_K2020051BBa_K2020051 Version 1 (Component)wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G
BBa_K1441013BBa_K1441013 Version 1 (Component)DNA ligase from Escherichia coli with His-tag INSERT
BBa_K1323020BBa_K1323020 Version 1 (Component)oriV from the Staphylococcus aureus pSK41 plasmid clone 3
BBa_K1766008BBa_K1766008 Version 1 (Component)EnvZ_V5 osmoregulatory histidine kinase from <i>E.coli</i>.
BBa_K1766014BBa_K1766014 Version 1 (Component)EnvZ osmoregulatory histidine kinase from <i>E.coli.</i>
BBa_K1497197BBa_K1497197 Version 1 (Component)B0034-CHI - Chalcone Isomerase from Petunia with strong RBS
BBa_K1465203BBa_K1465203 Version 1 (Component)Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Synechococcus elongatus
BBa_J10065BBa_J10065 Version 1 (Component)LuxI from V. fischeri C0061 with LVA tag removed
BBa_K1968014BBa_K1968014 Version 1 (Component)Tcyc Phytobrick: cytochrome C gene transcriptional terminator from Saccharomyces cerevisiae
BBa_K294205BBa_K294205 Version 1 (Component)This is a coding sequence of heat shock protein from E.coli
BBa_K2092004BBa_K2092004 Version 1 (Component)alcR (incl RBS), ethanol-activated transcription factor from A. nidulans
BBa_K1968009BBa_K1968009 Version 1 (Component)PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger
BBa_K1968013BBa_K1968013 Version 1 (Component)PgdaA constituve Phytobrick promoter: Glyceraldehyde-3-phosphate dehydrogenase from Aspergillus nige
BBa_K177035BBa_K177035 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA) under control of RBS.3 (medium)
BBa_K1441012BBa_K1441012 Version 1 (Component)DNA ligase from Escherichia coli with His-tag In pGAPz alpha A
Bacillus subtilis Collectionbsu_collection Version 1 (Collection)This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.