BBa_K228827
BBa_K228827 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(B0030)+T7ptag

BBa_K228829
BBa_K228829 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(B0033)+T7ptag

BBa_K228853
BBa_K228853 Version 1 (Component)
AND GATE Sal+SupD+LacP+RBS(B0030)+T7ptag

BBa_K1341010
BBa_K1341010 Version 1 (Component)
OUTPUT SYS(GFP)LOGIC GATE IN Graph Theory 2

BBa_K235026
BBa_K235026 Version 1 (Component)
[K235022][K235001] (NAND gate control test, pLac positive control)

BBa_K235027
BBa_K235027 Version 1 (Component)
[K235024][K235000] (NAND gate control test, arabinose positive control)

BBa_J34100
BBa_J34100 Version 1 (Component)
AND gate, 2x PoPS input, 1x PoPS output

BBa_K1132003
BBa_K1132003 Version 1 (Component)
AND gate with recombinases switching gene regulatory sequences and ORF

BBa_K1132004
BBa_K1132004 Version 1 (Component)
XOR gate with recombinases switching gene regulatory sequences and ORF

BBa_J34200
BBa_J34200 Version 1 (Component)
XOR gate, 2x PoPS input, 1x PoPS output

BBa_K145274
BBa_K145274 Version 1 (Component)
LuxI generator controlled by a AND-gate system.

BBa_J119136
BBa_J119136 Version 1 (Component)
Device for Testing New Promoters via Golden Gate Assembly

BBa_J119141
BBa_J119141 Version 1 (Component)
Device for Testing New Promoters via BsgI Golden Gate Assembly

BBa_K1994017
BBa_K1994017 Version 1 (Component)
sgRNA with 5' golden gate adapter and PP7 protein binding site

BBa_K1994015
BBa_K1994015 Version 1 (Component)
sgRNA with 5' golden gate adapter and COM protein binding site

BBa_K1341011
BBa_K1341011 Version 1 (Component)
OR LOGIC GATE IN Graph Theory (GFP OUTPUT DEVICE)

BBa_K1790001
BBa_K1790001 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790005
BBa_K1790005 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790000
BBa_K1790000 Version 1 (Component)
biosensors detect food allergens. A proteins conformation change in response to ligand binding coupl

BBa_K1790003
BBa_K1790003 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790004
BBa_K1790004 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790002
BBa_K1790002 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_J119313
BBa_J119313 Version 1 (Component)
pClone Blue - Device for Testing New Promoters via Golden Gate Assembly

BBa_J119137
BBa_J119137 Version 1 (Component)
pClone Red - Device for Testing New Promoters via Golden Gate Assembly

BBa_K1994016
BBa_K1994016 Version 1 (Component)
sgRNA with 5' golden gate adapter and MS2 coat protein binding site

BBa_M39023
BBa_M39023 Version 1 (Component)
AND gate structure for regulation of E.coli in CF mucus

BBa_J119367
BBa_J119367 Version 1 (Component)
tClone: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly

BBa_K1649002
BBa_K1649002 Version 1 (Component)
Red light sensor with TetR not gate and GFP reporter

BBa_K812051
BBa_K812051 Version 1 (Component)
Golden brick version of pSB1K3 plasmid for Golden Gate and Golden Brick cloning

BBa_J119142
BBa_J119142 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

BBa_J119361
BBa_J119361 Version 1 (Component)
tClone Red: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly

pSB1C3 GB
BBa_K812050 Version 1 (Component)
Golden brick version of pSB1C3 plasmid for Golden Gate and Golden Brick cloning

BBa_J119374
BBa_J119374 Version 1 (Component)
tClone TetA: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly

BBa_K235025
BBa_K235025 Version 1 (Component)
[K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal)

BBa_K2116028
BBa_K2116028 Version 1 (Component)
bxb1 integrase under the control of nitric oxide and AHL responsive AND gate

BBa_K2116068
BBa_K2116068 Version 1 (Component)
AND gate regulated by norR and esaR (internal esabox, overlapping with sigma 54 binding site, before

BBa_K1132037
BBa_K1132037 Version 1 (Component)
AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter

BBa_K1132038
BBa_K1132038 Version 1 (Component)
XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter

BBa_K1903003
BBa_K1903003 Version 1 (Component)
Golden Gate Adapter

BBa_M41008
BBa_M41008 Version 1 (Component)
AND Gate Promoter

BBa_K2116004
BBa_K2116004 Version 1 (Component)
AND gate regulated by norR and esaR (one esabox)

BBa_K1363604
BBa_K1363604 Version 1 (Component)
key of no of RNA logic gates

BBa_K228013
BBa_K228013 Version 1 (Component)
(pSal PO) OR Gate - GFP

BBa_J54141
BBa_J54141 Version 1 (Component)
AND GATE"AHL and oleate"

BBa_K2116006
BBa_K2116006 Version 1 (Component)
AND gate regulated by norR and esaR (three esaboxes)

BBa_J119300
BBa_J119300 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.