BBa_K113014
BBa_K113014 Version 1 (Component)
truncated T7 promoter->GFP

BBa_K113015
BBa_K113015 Version 1 (Component)
bidirectional intact T7 promoters->GFP/RFP

BBa_K113016
BBa_K113016 Version 1 (Component)
overlapping T7 promoter->GFP/RFP

BBa_K113017
BBa_K113017 Version 1 (Component)
more overlapping T7 promoter->GFP/RFP

BBa_K113018
BBa_K113018 Version 1 (Component)
weaken overlapping T7 promoter->GFP/RFP

BBa_K113019
BBa_K113019 Version 1 (Component)
pBad/araC->T7 polymerase->GFP

BBa_K113020
BBa_K113020 Version 1 (Component)
GFP

BBa_K113021
BBa_K113021 Version 1 (Component)
Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter

mtGFP
BBa_K809001 Version 1 (Component)
Mitochondrial GFP

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_K098999
BBa_K098999 Version 1 (Component)
mtrB with RBS

BBa_K098998
BBa_K098998 Version 1 (Component)
cIts coding region with rbs

BBa_K098996
BBa_K098996 Version 1 (Component)
mtrB with terminator

BBa_K098994
BBa_K098994 Version 1 (Component)
mtrB with RBS and terminator

BBa_K098995
BBa_K098995 Version 1 (Component)
heat sensitive cI QPI with high promoter

BBa_K098993
BBa_K098993 Version 1 (Component)
heat sensitive cI QPI with low promoter

BBa_K098991
BBa_K098991 Version 1 (Component)
cI promoter with GFP reporter

BBa_S04138
BBa_S04138 Version 1 (Component)
cI QPI with GFP reporter system

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

pRecA
BBa_K093000 Version 1 (Component)
pRecA with LexA binding site

BBa_J24815
BBa_J24815 Version 1 (Component)
J23101 promoter with Renilla gene attached

BBa_J24816
BBa_J24816 Version 1 (Component)
J23101 promoter with Luc gene attached

BBa_K102021
BBa_K102021 Version 1 (Component)
TA0 gate in output test harness

BBa_K131026
BBa_K131026 Version 1 (Component)
AHL inducible GFP

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_K143071
BBa_K143071 Version 1 (Component)
AmyE integratable PoPS generator (P43-gsiB) (with CmR)

BBa_K143072
BBa_K143072 Version 1 (Component)
AmyE integratable PoPS generator (P43-spoVG) (with CmR)

BBa_K143073
BBa_K143073 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)

BBa_K143074
BBa_K143074 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)

BBa_J69522
BBa_J69522 Version 1 (Component)
tetR-repressible promoter coupled with mCherry Reporter

BBa_K102031
BBa_K102031 Version 1 (Component)
TA1 gate in output test harness

BBa_K102041
BBa_K102041 Version 1 (Component)
TA2 gate in output test harness

BBa_K102051
BBa_K102051 Version 1 (Component)
TA3 gate in output test harness

BBa_K102061
BBa_K102061 Version 1 (Component)
TA6 gate in output test harness

BBa_K102071
BBa_K102071 Version 1 (Component)
TA7 gate in output test harness

BBa_K102081
BBa_K102081 Version 1 (Component)
TA8 gate in output test harness

BBa_K102091
BBa_K102091 Version 1 (Component)
TA9 gate in output test harness

BBa_K102101
BBa_K102101 Version 1 (Component)
TA10 gate in output test harness

BBa_K102111
BBa_K102111 Version 1 (Component)
TA11 gate in output test harness

BBa_K102121
BBa_K102121 Version 1 (Component)
TA12 gate in output test harness

BBa_K102131
BBa_K102131 Version 1 (Component)
TA13 gate in output test harness

BBa_K102141
BBa_K102141 Version 1 (Component)
TA14 gate in output test harness

BBa_K143076
BBa_K143076 Version 1 (Component)
B. subtilis AmyE locus RFP output promoter and RBS tester

BBa_K143075
BBa_K143075 Version 1 (Component)
B. subtilis AmyE locus GFP output promoter and RBS tester

BBa_K143077
BBa_K143077 Version 1 (Component)
P43-gsiB GFP expression construct

BBa_K143078
BBa_K143078 Version 1 (Component)
P43-spoVG GFP expression construct

BBa_K143079
BBa_K143079 Version 1 (Component)
Pveg-spoVG GFP expression construct