BBa_K931003
BBa_K931003 Version 1 (Component)
An L-Arabinose Controlled Vitamin B12 Binding - Starch Binding Protein Fusion

BBa_K078002
BBa_K078002 Version 1 (Component)
A fragment that encodes an enzyme involed in dioxin degradation, the third step

BBa_K1796201
BBa_K1796201 Version 1 (Component)
An unloaded sgRNA that contains BbsI cutting site, with a promoter and terminator.

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

BBa_K2007003
BBa_K2007003 Version 1 (Component)
Constitutive promoter, an RBS, and Drosophila melanogaster Juvenile Hormone Acid O-methyltransferase

BBa_K2122000
BBa_K2122000 Version 1 (Component)
Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter

BBa_K1678007
BBa_K1678007 Version 1 (Component)
Vitamin B12 biosensor, composed by a cobalamin riboswitch in front of an mRFP1

VVD-YC
BBa_K1616021 Version 1 (Component)
VVD linked to YC155 (YFP Cter split)

BBa_K1431101
BBa_K1431101 Version 1 (Component)
TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA

BBa_K1985013
BBa_K1985013 Version 1 (Component)
Sequence coding for Sup35 with residues 1-61 with an arabinose inducible promoter

BBa_K2082232
BBa_K2082232 Version 1 (Component)
RFP under the control of an optimized lacZ promoter combined with Gal11P:cI(434)

BBa_K525161
BBa_K525161 Version 1 (Component)
Fusion Protein of Luciferase and cspB from Corynebacterium glutamicum with TAT-Sequence and lipid an

BBa_K557000
BBa_K557000 Version 1 (Component)
CheZ: phosphatase which dephosphorylates CheY-P and thus plays an important role in E.Coli chemotaxi

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

BBa_K2007004
BBa_K2007004 Version 1 (Component)
Fatty acid sensitive promoter, an RBS, and Drosophila melanogaster Juvenile Hormone Acid O-methyltra

BBa_K1179017
BBa_K1179017 Version 1 (Component)
A mutant DNA binding Cas9 with an N-terminal linker and BsaI site for ligation

BBa_J04795
BBa_J04795 Version 1 (Component)
Riboswitch designed to turn "ON" a protein

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_K1985014
BBa_K1985014 Version 1 (Component)
Sequence coding for Sup35(residues 1-61) and Cytochrome b562 with an arabinose inducible promoter

BBa_K2082231
BBa_K2082231 Version 1 (Component)
RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434

BBa_K936012
BBa_K936012 Version 1 (Component)
Leader sequence that brings protein to periplasm

BBa_K1861020
BBa_K1861020 Version 1 (Component)
Fusion protein of the TALE constant domain and a SpyTag in an expression cassette

BBa_I733004
BBa_I733004 Version 1 (Component)
Produce LacZ alpha in response to AHL

BBa_K185033
BBa_K185033 Version 1 (Component)
An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31

BBa_S03766
BBa_S03766 Version 1 (Component)
RBS-Kan-RBS-Tet-RBS-RFP (pSB1A7)

BBa_K1462400
BBa_K1462400 Version 1 (Component)
pGAL1+PRK+GBD-ligand+ADH1+pTDH3+RuBisCo+SH3-ligand+ADH1+pTDH3+CA+PDZ-ligand+ADH1

BBa_K1615108
BBa_K1615108 Version 1 (Component)
mRFP fused to CBDclos driven by LacI promoter

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K1657006
BBa_K1657006 Version 1 (Component)
It is called GAB. It have the resistance to glyphosate and glufosinate

[OriTR]+[R
BBa_K1439002 Version 1 (Component)
This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K541715
BBa_K541715 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide

BBa_K1961007
BBa_K1961007 Version 1 (Component)
CYP1A2, an enzyme of cytochrome P450 protein family that metabolizes toxin in liver