BBa_K917010
BBa_K917010 Version 1 (Component)
Kanamycin resistance+lac promoter+RFP+sacB selection-counterselection cassette

BBa_K1381014
BBa_K1381014 Version 1 (Component)
Silencing sRNA (10 bases long) for USP45 (Based on Spot42)

BBa_K1381016
BBa_K1381016 Version 1 (Component)
Silencing sRNA (20 bases long) for USP45 (Based on Spot42)

BBa_K1381015
BBa_K1381015 Version 1 (Component)
Silencing sRNA (15 bases long) for USP45 (Based on Spot42)

BBa_K1797016
BBa_K1797016 Version 1 (Component)
dCas9-Long Linker (Gly-Gly-Ser-Gly)x3-Flp

BBa_K615004
BBa_K615004 Version 1 (Component)
Zif268 binding site-His3-URA3 construct for one-hybrid selection

BBa_K615000
BBa_K615000 Version 1 (Component)
E. coli strain for His3-URA3 one-hybrid selection system

BBa_J58120
BBa_J58120 Version 1 (Component)
OmpR-P detector with a RFP pulse generator at intermediate concentrations

BBa_J119372
BBa_J119372 Version 1 (Component)
Ptac mutant -34 TT Deletion and -28 GGC Insertion

BBa_K1507001
BBa_K1507001 Version 1 (Component)
Long-chain fatty acid outer membrane transporter (J23119-B0034-FadL-B0015)

BBa_M36055
BBa_M36055 Version 1 (Component)
DNA damage detector with Gemini Reporter (No LexA Generator)

BBa_K883051
BBa_K883051 Version 1 (Component)
CCMV coat protein 25 AA N terminal deletion with IPTG induced promoter

BBa_K1945002
BBa_K1945002 Version 1 (Component)
Homologous Recombination Intermediate for Counter-selection in Synechocystis sp. PCC 6803

BBa_K524203
BBa_K524203 Version 1 (Component)
pSC101 heat sensitive origin of replication with deletion in par locus

BBa_K1790000
BBa_K1790000 Version 1 (Component)
biosensors detect food allergens. A proteins conformation change in response to ligand binding coupl

BBa_K1797015
BBa_K1797015 Version 1 (Component)
dCas9-Long Linker (Gly-Gly-Ser-Gly)x3-Bxb1 gp35

BBa_M41009
BBa_M41009 Version 1 (Component)
Genome sequence of long direct repeat sequence - D which encodes LdrD.

BBa_K1437008
BBa_K1437008 Version 1 (Component)
mouse AhR detective protein 521-805 without terminator codon optimized for yeast

BBa_K1437006
BBa_K1437006 Version 1 (Component)
mouse AhR detective protein 83-805 with terminator codon optimized for yeast

BBa_K1437007
BBa_K1437007 Version 1 (Component)
mouse AhR detective protein 521-805 with terminator codon optimized for yeast

BBa_K891002
BBa_K891002 Version 1 (Component)
Probe coding for alpha fragment of beta gal (long linker)

BBa_K891006
BBa_K891006 Version 1 (Component)
Probe coding for alpha fragment of beta gal (long linker)

BBa_M45102
BBa_M45102 Version 1 (Component)
Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese

BBa_K648016
BBa_K648016 Version 1 (Component)
Fast-Fusion GFP-Xyle Reporter (TEV cleaveage with long linker)

BBa_K648025
BBa_K648025 Version 1 (Component)
Fast-Fusion GFP-LacZa Reporter (RecA cleavage with Long linker)

BBa_K648022
BBa_K648022 Version 1 (Component)
Fast-Fusion GFP-Xyle Reporter (RecA cleaveage with long linker)

BBa_K648019
BBa_K648019 Version 1 (Component)
Fast-Fusion GFP-LacZa Reporter (TEV cleavage with long linker)

IBMc386
IBMc386 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-171)-LNG-M86N-cat-Plux2-B32-M86C-S-mCherry*(172-236)-H6-T

BBa_K1698003
BBa_K1698003 Version 1 (Component)
BaseHunter : Activated to become detector for 32bp target sequence on SRY Gene

BBa_K1698004
BBa_K1698004 Version 1 (Component)
BaseHunter : Activated to become detector for 24bp target sequence on Mycobacterium genomes

BBa_K1698002
BBa_K1698002 Version 1 (Component)
BaseHunter : Activated to become detector for 62bp target sequence on SRY gene

BBa_K1725347
BBa_K1725347 Version 1 (Component)
<i>luxG</i> with optimised RBS for expression in <i>E. coli</i>

BBa_J119336
BBa_J119336 Version 1 (Component)
Ammeline Biosensor with Riboswitch M6C prime

BBa_J24802
BBa_J24802 Version 1 (Component)
Lead Detector Gene

BBa_K675002
BBa_K675002 Version 1 (Component)
Arsenic Detector

BBa_K1452000
BBa_K1452000 Version 1 (Component)
Pos/neg selection (Bacillus)

BBa_K1366101
BBa_K1366101 Version 1 (Component)
Genetic construct for lpp gene deletion (C1)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.